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PMID: 2236077 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Enzymatic aminoacylation of an eight-base-pair microhelix with histidine.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 21 ·1990-11-00 ·Pages 8655-9

Francklyn C, Schimmel P

Abstract

The major determinant for the identity of alanine tRNAs is a single base pair in the acceptor helix that is proximal to the site of amino acid attachment. A 7-base-pair microhelix that recreates the acceptor helix can be charged with alanine. No other examples of charging of small helices with specific amino acids have been reported, to our knowledge. We show here that a 13-base-pair and an 8-base-pair hairpin helix that reconstruct a domain and subdomain, respectively, of histidine tRNAs can be charged with histidine. We also show that transplantation of a base pair that is unique to histidine tRNAs is sufficient to consider histidine acceptance on a domain and subdomain of alanine tRNA. Both alanine and histidine aminoacyl-tRNA synthetases retain specificity for their cognate synthetic substrates. Alanine- and histidine-specific microhelices may resemble a system that arose early in the evolution of charging and coding.

MeSH Terms
Alanine-tRNA Ligase/metabolism Base Composition Base Sequence Histidine-tRNA Ligase/metabolism Kinetics Models, Molecular Molecular Sequence Data Nucleic Acid Conformation RNA, Transfer, Ala/genetics,metabolism RNA, Transfer, His/genetics,metabolism Substrate Specificity
Chemicals
RNA, Transfer, Ala RNA, Transfer, His Histidine-tRNA Ligase Alanine-tRNA Ligase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Francklyn C
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Schimmel P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.21.8655
Published
1990-11-00
Pages
8655-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC55016
Subset
IM
Grants
NIGMS NIH HHS · GM 15539 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.21.8655 PMC Full Text View journal details
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