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PMID: 2236065 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

ISGF3, the transcriptional activator induced by interferon alpha, consists of multiple interacting polypeptide chains.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 21 ·1990-11-00 ·Pages 8555-9

Fu XY, Kessler DS, Veals SA, Levy DE, Darnell JE

Abstract

Interferon-stimulated gene factor 3 (ISGF3) is the ligand-dependent transcriptional activator that, in response to interferon treatment, is assembled in the cell cytoplasm, is translocated to the nucleus, and binds the consensus DNA site, the interferon-stimulated response element. We have purified ISGF3 and identified its constituent proteins: a DNA-binding protein of 48 kDa and three larger polypeptides (84, 91, and 113 kDa), which themselves do not have DNA-binding activity. The multisubunit structure of ISGF3 most likely reflects its participation in receiving a ligand-dependent signal, translocating to the nucleus, and binding to DNA to activate transcription.

MeSH Terms
Base Sequence Chromatography, Affinity Chromatography, Ion Exchange DNA DNA-Binding Proteins/isolation & purification,metabolism HeLa Cells/drug effects,metabolism Humans Interferon Type I/pharmacology Interferon-Stimulated Gene Factor 3 Interferon-Stimulated Gene Factor 3, gamma Subunit Macromolecular Substances Molecular Sequence Data Molecular Weight Oligodeoxyribonucleotides Transcription Factors/isolation & purification,metabolism Transcription, Genetic
Chemicals
DNA-Binding Proteins IRF9 protein, human Interferon Type I Interferon-Stimulated Gene Factor 3 Interferon-Stimulated Gene Factor 3, gamma Subunit Macromolecular Substances Oligodeoxyribonucleotides Transcription Factors DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Fu X Y
Laboratory of Molecular Cell Biology, Rockefeller University, New York, NY 10021.
Kessler D S
Veals S A
Levy D E
Darnell J E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.21.8555
Published
1990-11-00
Pages
8555-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54995
Subset
IM
Grants
NIAID NIH HHS · AI07233-15 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.21.8555 PMC Full Text View journal details
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