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PMID: 2236008 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Induction of verotoxin sensitivity in receptor-deficient cell lines using the receptor glycolipid globotriosylceramide.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 20 ·1990-10-00 ·Pages 7898-901

Waddell T, Cohen A, Lingwood CA

Abstract

Verotoxin 1 is an Escherichia coli-derived subunit toxin that specifically binds to the glycolipid globotriosylceramide and is cytotoxic for cells that contain this plasma membrane glycolipid. Glycolipid incorporation experiments have now been performed using human lymphoid cells of the B lineage that lack this receptor, to conclusively demonstrate that globotriosylceramide alone is a functional receptor for this toxin. Globotriosylceramide incorporated into the membrane of toxin-resistant cells provides intracellular access to verotoxin by receptor-mediated endocytosis. Protein synthesis is then inhibited and globotriosylceramide-containing cells are killed.

MeSH Terms
Bacterial Toxins/metabolism,pharmacology Cell Line Cell Survival/drug effects Escherichia coli Humans Kinetics Liposomes Macromolecular Substances Methionine/metabolism Protein Biosynthesis Receptors, Cell Surface Receptors, Immunologic/genetics,metabolism Shiga Toxin 1 Trihexosylceramides/metabolism
Chemicals
Bacterial Toxins Liposomes Macromolecular Substances Receptors, Cell Surface Receptors, Immunologic Shiga Toxin 1 Trihexosylceramides shigella toxin receptor globotriaosylceramide Methionine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Waddell T
Department of Microbiology, Hospital for Sick Children, Toronto, Ontario, Canada.
Cohen A
Lingwood C A
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.20.7898
Published
1990-10-00
Pages
7898-901
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54858
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.87.20.7898 PMC Full Text View journal details
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