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PMID: 22332661 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Nasal cell DNA methylation, inflammation, lung function and wheezing in children with asthma.

Epigenomics ·Vol. 4 ·No. 1 ·2012-02-00 ·Pages 91-100

Baccarelli A, Rusconi F, Bollati V, Catelan D, Accetta G, Hou L, Barbone F, Bertazzi PA, Biggeri A

Abstract

DNA methylation is increasingly proposed as a mechanism for underlying asthma-related inflammation. However, epigenetic studies are constrained by uncertainties on whether samples that can be easily collected in human individuals can provide informative results. Two nasal cell DNA samples were collected on different days by nasal brushings from 35 asthmatic children aged between 8 and 11 years old. We correlated DNA methylation of IL-6, iNOS, Alu and LINE-1 with fractional exhaled nitric oxide, forced expiratory volume in 1 s and wheezing. Fractional exhaled nitric oxide increased in association with lower promoter methylation of both IL-6 (+29.0%; p = 0.004) and iNOS (+41.0%; p = 0.002). Lower IL-6 methylation was nonsignificantly associated with wheezing during the week of the study (odds ratio = 2.3; p = 0.063). Our findings support the use of nasal cell DNA for human epigenetic studies of asthma.

MeSH Terms
Alu Elements/genetics Asthma/complications,genetics,metabolism Child DNA Methylation Epigenesis, Genetic Female Humans Inflammation/etiology,genetics Interleukin-6/genetics,metabolism Long Interspersed Nucleotide Elements/genetics Lung/physiopathology Male Nasal Mucosa/cytology,metabolism Nitric Oxide/metabolism Nitric Oxide Synthase Type II/genetics,metabolism Promoter Regions, Genetic Respiratory Sounds/etiology,genetics
Chemicals
Interleukin-6 Nitric Oxide Nitric Oxide Synthase Type II
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Baccarelli Andrea
Exposure Epidemiology & Risk Program, Harvard School of Public Health, Boston, MA 02215, USA. abaccare@hsph.harvard.edu
Rusconi Franca
Bollati Valentina
Catelan Dolores
Accetta Gabriele
Hou Lifang
Barbone Fabio
Bertazzi Pier Alberto
Biggeri Annibale
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Article Info
Journal
Epigenomics
Abbr.
Epigenomics
ISSN
1750-192X
DOI
10.2217/epi.11.106
Email
abaccare@hsph.harvard.edu
Published
2012-02-00
Pages
91-100
Language
English
Region
England
NLM ID
101519720
PMCID
PMC3297414
Subset
IM
Grants
NIEHS NIH HHS · P30 ES000002 · United States
NIEHS NIH HHS · P30 ES000002-49 · United States
NIEHS NIH HHS · P30ES000002 · United States
External Links
PubMed source DOI: 10.2217/epi.11.106 PMC Full Text View journal details
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