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PMID: 22312431 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Coronavirus papain-like proteases negatively regulate antiviral innate immune response through disruption of STING-mediated signaling.

PloS one ·Vol. 7 ·No. 2 ·2012-00-00 ·Pages e30802

Sun L, Xing Y, Chen X, Zheng Y, Yang Y, Nichols DB, Clementz MA, Banach BS, Li K, Baker SC, Chen Z

Abstract

Viruses have evolved elaborate mechanisms to evade or inactivate the complex system of sensors and signaling molecules that make up the host innate immune response. Here we show that human coronavirus (HCoV) NL63 and severe acute respiratory syndrome (SARS) CoV papain-like proteases (PLP) antagonize innate immune signaling mediated by STING (stimulator of interferon genes, also known as MITA/ERIS/MYPS). STING resides in the endoplasmic reticulum and upon activation, forms dimers which assemble with MAVS, TBK-1 and IKKε, leading to IRF-3 activation and subsequent induction of interferon (IFN). We found that expression of the membrane anchored PLP domain from human HCoV-NL63 (PLP2-TM) or SARS-CoV (PLpro-TM) inhibits STING-mediated activation of IRF-3 nuclear translocation and induction of IRF-3 dependent promoters. Both catalytically active and inactive forms of CoV PLPs co-immunoprecipitated with STING, and viral replicase proteins co-localize with STING in HCoV-NL63-infected cells. Ectopic expression of catalytically active PLP2-TM blocks STING dimer formation and negatively regulates assembly of STING-MAVS-TBK1/IKKε complexes required for activation of IRF-3. STING dimerization was also substantially reduced in cells infected with SARS-CoV. Furthermore, the level of ubiquitinated forms of STING, RIG-I, TBK1 and IRF-3 are reduced in cells expressing wild type or catalytic mutants of PLP2-TM, likely contributing to disruption of signaling required for IFN induction. These results describe a new mechanism used by CoVs in which CoV PLPs negatively regulate antiviral defenses by disrupting the STING-mediated IFN induction.

MeSH Terms
Adaptor Proteins, Signal Transducing/metabolism Animals Biocatalysis Cell Membrane/enzymology Chlorocebus aethiops Coronavirus 3C Proteases Coronavirus NL63, Human/enzymology,physiology Cysteine Endopeptidases/chemistry,metabolism HEK293 Cells Humans I-kappa B Kinase/metabolism Immunity, Innate Interferon Regulatory Factor-3/metabolism Interferons/metabolism Membrane Proteins/chemistry,metabolism Protein Multimerization Protein Structure, Quaternary Protein Structure, Tertiary SARS Virus/enzymology,physiology Signal Transduction/immunology Ubiquitination/immunology Vero Cells Viral Proteins/chemistry,metabolism
Chemicals
Adaptor Proteins, Signal Transducing Interferon Regulatory Factor-3 MAVS protein, human Membrane Proteins STING1 protein, human Viral Proteins Interferons I-kappa B Kinase Cysteine Endopeptidases Coronavirus 3C Proteases
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Sun Li
Division of Infection and Immunity, Department of Electromagnetic and Laser Biology, Beijing Institute of Radiation Medicine, Beijing, China.
Xing Yaling
Chen Xiaojuan
Zheng Yang
Yang Yudong
Nichols Daniel B
Clementz Mark A
Banach Bridget S
Li Kui
Baker Susan C
Chen Zhongbin
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
Published
2012-00-00
Epub
2012-00-01
Pages
e30802
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC3270028
Subset
IM
Grants
NIAID NIH HHS · P01 AI060915 · United States
NIAID NIH HHS · R01 AI085089 · United States
NIAID NIH HHS · AI060915 · United States
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