Differentiation of genes coding for Escherichia coli verotoxin 2 and the verotoxin associated with porcine edema disease (VTe) by the polymerase chain reaction.

Journal of clinical microbiology ·Vol. 28 ·No. 10 ·1990-10-00 ·Pages 2351-3

Johnson WM, Pollard DR, Lior H, Tyler SD, Rozee KR

Abstract

Two sets of synthetic oligonucleotide primers were used in a polymerase chain reaction adaptation to distinguish the closely related genes for type 2 verotoxin (VT2 or Shiga-like toxin [SLT-II]) and the verotoxin associated with porcine edema disease (VTe or SLT-II variant [SLT-IIv]) in Escherichia coli.

MeSH Terms

Animals Bacterial Toxins/genetics DNA Probes Edema/etiology,veterinary Escherichia coli/genetics Escherichia coli Infections/etiology,veterinary Genes, Bacterial Polymerase Chain Reaction Shiga Toxin 1 Swine Swine Diseases/etiology

Chemicals

Bacterial Toxins DNA Probes Shiga Toxin 1

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Johnson W M

National Laboratory for Enteric Pathogens, Laboratory Centre for Disease Control, Ottawa, Ontario, Canada.

Pollard D R

Lior H

Tyler S D

Rozee K R

References (13)

13 references, click to expand

New biological effect of edema disease principle (Escherichia coli-neurotoxin) and its use as an in vitro for this toxin.
Am J Vet Res. 1983 Jan;44(1):31-4 PMID: 6337536
Infection by verocytotoxin-producing Escherichia coli.
Clin Microbiol Rev. 1989 Jan;2(1):15-38 PMID: 2644022
Cloning and nucleotide sequence analysis of the genes determining verocytotoxin production in a porcine edema disease isolate of Escherichia coli.
Microb Pathog. 1988 Dec;5(6):419-26 PMID: 3072464
Purification and some properties of a Vero toxin from a human strain of Escherichia coli that is immunologically related to Shiga-like toxin II (VT2).
Microb Pathog. 1989 Feb;6(2):113-22 PMID: 2654533
Synthetic oligodeoxyribonucleotide probes to detect verocytotoxin-producing Escherichia coli in diseased pigs.
FEMS Microbiol Lett. 1989 Jan 15;48(2):247-52 PMID: 2656385
Evaluation of oligonucleotide probes for identification of shiga-like-toxin-producing Escherichia coli.
J Clin Microbiol. 1989 Jun;27(6):1180-6 PMID: 2666436
A new heat-labile cytolethal distending toxin (CLDT) produced by Escherichia coli isolates from clinical material.
Microb Pathog. 1988 Feb;4(2):103-13 PMID: 2849027
Hybridization of Escherichia coli producing Shiga-like toxin I, Shiga-like toxin II, and a variant of Shiga-like toxin II with synthetic oligonucleotide probes.
Infect Immun. 1989 Sep;57(9):2811-4 PMID: 2668189
Rapid and specific detection of verotoxin genes in Escherichia coli by the polymerase chain reaction.
J Clin Microbiol. 1990 Mar;28(3):540-5 PMID: 2182671
Verotoxin production among porcine strains of Escherichia coli and its association with oedema disease.
J Med Microbiol. 1987 Dec;24(4):359-62 PMID: 3320373
Cloning and sequencing of a Shiga-like toxin type II variant from Escherichia coli strain responsible for edema disease of swine.
J Bacteriol. 1988 Sep;170(9):4223-30 PMID: 3045088
DNA probes for Shiga-like toxins I and II and for toxin-converting bacteriophages.
J Clin Microbiol. 1988 Jul;26(7):1292-7 PMID: 2842369
Detection and identification of pathotypes of verocytotoxigenic Escherichia coli isolated from weaned piglets using gene probes for seven E. coli toxins.
FEMS Microbiol Lett. 1989 Jun;50(3):345-9 PMID: 2668112

Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.28.10.2351-2353.1990

Published

1990-10-00

Pages

2351-3

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC268176

Subset

External Links

PubMed source DOI: 10.1128/jcm.28.10.2351-2353.1990 PMC Full Text View journal details

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Differentiation of genes coding for Escherichia coli verotoxin 2 and the verotoxin associated with porcine edema disease (VTe) by the polymerase chain reaction.

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