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PMID: 22274765 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

SSR-based genetic maps of Miscanthus sinensis and M. sacchariflorus, and their comparison to sorghum.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik ·Vol. 124 ·No. 7 ·2012-05-00 ·Pages 1325-38

Kim C, Zhang D, Auckland SA, Rainville LK, Jakob K, Kronmiller B, Sacks EJ, Deuter M, Paterson AH

Abstract

We present SSR-based genetic maps from a cross between Miscanthus sacchariflorus Robustus and M. sinensis, the progenitors of the promising cellulosic biofuel feedstock Miscanthus × giganteus. cDNA-derived SSR markers were mapped by the two-way pseudo-testcross model due to the high heterozygosity of each parental species. A total of 261 loci were mapped in M. sacchariflorus, spanning 40 linkage groups and 1,998.8 cM, covering an estimated 72.7% of the genome. For M. sinensis, a total of 303 loci were mapped, forming 23 linkage groups and 2,238.3 cM, covering 84.9% of the genome. The use of cDNA-derived SSR loci permitted alignment of the Miscanthus linkage groups to the sorghum chromosomes, revealing a whole genome duplication affecting the Miscanthus lineage after the divergence of subtribes Sorghinae and Saccharinae, as well as traces of the pan-cereal whole genome duplication. While the present maps provide for many early research needs in this emerging crop, additional markers are also needed to improve map density and to further characterize the structural changes of the Miscanthus genome since its divergence from sorghum and Saccharum.

MeSH Terms
Chromosome Mapping/methods DNA, Plant/genetics Genetic Linkage Genetic Markers Microsatellite Repeats Saccharum/genetics Sequence Alignment Sorghum/genetics
Chemicals
DNA, Plant Genetic Markers
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Kim Changsoo
Plant Genome Mapping Laboratory, University of Georgia, 111 Riverbend Road, Rm 228, Athens, GA 30602, USA.
Zhang Dong
Auckland Susan A
Rainville Lisa K
Jakob Katrin
Kronmiller Brent
Sacks Erik J
Deuter Martin
Paterson Andrew H
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Article Info
Journal
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Abbr.
Theor Appl Genet
ISSN
1432-2242
DOI
10.1007/s00122-012-1790-1
Published
2012-05-00
Epub
2012-00-25
Pages
1325-38
Language
English
Region
Germany
NLM ID
0145600
Subset
IM
External Links
PubMed source DOI: 10.1007/s00122-012-1790-1 View journal details
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