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PMID: 222669 Published · ppublish English Journal Article

Organ culture of adult mouse intestine. I. Morphological results after 24 and 48 hours of culture.

In vitro ·Vol. 15 ·No. 4 ·1979-04-00 ·Pages 278-87

Ferland S, Hugon JS

Abstract

Explants of adult mouse intestine have been maintained in organ culture for 24 to 48 hr. The best results have been obtained with a mixture of DMEM-HEPES medium and NCTC-135 enriched with 10% fetal bovine serum. The morphology of the mucosa is well preserved at the light and electron microscopic level: absorbing cells exhibit an increase in secondary lysosomes; goblet cells and Paneth cells remain active; numerous mitoses are observed in the crypts; and vigorous re-epithelization takes place on the margin of the explants.

MeSH Terms
Animals Cell Differentiation Culture Media Duodenum/cytology Intestinal Mucosa/cytology Jejunum/cytology Lymphocytes/cytology Male Mice Mice, Inbred ICR Microvilli/ultrastructure Mitosis Organ Culture Techniques Organoids/ultrastructure Phosphoric Monoester Hydrolases/analysis
Chemicals
Culture Media Phosphoric Monoester Hydrolases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ferland S
Hugon J S
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Article Info
Journal
In vitro
Abbr.
In Vitro
ISSN
0073-5655
DOI
10.1007/BF02618952
Published
1979-04-00
Pages
278-87
Language
English
Region
United States
NLM ID
0063733
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02618952
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