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PMID: 22232680 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Phytochrome regulates translation of mRNA in the cytosol.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 109 ·No. 4 ·2012-01-24 ·Pages 1335-40

Paik I, Yang S, Choi G

Abstract

An array of photoreceptors including cryptochromes, phototropin, and phytochromes regulates various light responses in plants. Among these photoreceptors, phytochromes perceive red and far-red light by switching between two interconvertible spectral forms (Pr and Pfr). The Pfr form promotes light responses partly by destabilizing negatively acting, phytochrome-interacting basic helix-loop-helix transcription factors (PIFs), thus modulating transcription in the nucleus. The Pfr form is also present in the cytosol. However, the role of phytochromes in the cytosol is not well understood. Here we show that the Pfr form interacts with the cytosolic protein PENTA1 (PNT1) and inhibits the translation of protochlorophyllide reductase (PORA) mRNA. PNT1 possesses five C3H-type zinc finger domains and displays similarity to various RNA binding proteins including Tristetraprolin, which regulates stabilities of mRNAs such as TNF-α mRNA in humans. Consistent with its function as an RNA binding protein, PNT1 directly binds to mRNA of a key chlorophyll biosynthetic gene, protochlorophyllide reductase in vivo and inhibits the translation of PORA mRNA in the presence of phytochromes. The present results demonstrate that phytochromes transmit light signals to regulate not only transcription in the nucleus through PIFs, but also translation in the cytosol through PNT1.

MeSH Terms
Arabidopsis/physiology Arabidopsis Proteins/genetics,metabolism Cluster Analysis Cytosol/metabolism DNA Primers/genetics Gene Expression Regulation, Plant/genetics,physiology Luciferases Mannosyltransferases/genetics,metabolism Oxidoreductases Acting on CH-CH Group Donors/genetics,metabolism Photobleaching Phylogeny Phytochrome/metabolism,physiology RNA, Messenger/genetics,metabolism Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Two-Hybrid System Techniques
Chemicals
Arabidopsis Proteins DNA Primers RNA, Messenger Phytochrome Luciferases Oxidoreductases Acting on CH-CH Group Donors protochlorophyllide reductase Mannosyltransferases PNT1 protein, Arabidopsis
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Paik Inyup
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.
Yang Seungchan
Choi Giltsu
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.1109683109
Published
2012-01-24
Epub
2012-00-09
Pages
1335-40
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC3268310
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.1109683109 PMC Full Text View journal details
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