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PMID: 22162267 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

UM-SCC-104: a new human papillomavirus-16-positive cancer stem cell-containing head and neck squamous cell carcinoma cell line.

Head & neck ·Vol. 34 ·No. 10 ·2012-10-00 ·Pages 1480-91

Tang AL, Hauff SJ, Owen JH, Graham MP, Czerwinski MJ, Park JJ, Walline H, Papagerakis S, Stoerker J, McHugh JB, Chepeha DB, Bradford CR, Carey TE, Prince ME

Abstract

Few human papillomavirus (HPV)(+) head and neck squamous cell carcinoma (HNSCC) cell lines exist. We established University of Michigan-squamous cell carcinoma-104 (UM-SCC-104), a new HPV(+) HNSCC cell line from a recurrent oral cavity tumor, and characterized it for the presence of cancer stem cells (CSCs). Tumor cells were tested for biomarker expression by immunohistology, and the presence of HPV was assessed by several methods. UM-SCC-104 has a unique genotype, contains HPV-16, and expresses E6/E7. Inoculation of aldehyde dehydrogenase (ALDH)(+) and ALDH(-) cells in an immunocompromised mouse resulted in tumor growth from the ALDH(+) cells after 6 weeks that recapitulated the histology of the primary, whereas ALDH(-) cells did not produce tumors. UM-SCC-104, a new HPV-16, CSC-containing HNSCC cell line will aid in studying recurrent HPV(+) tumors. The aggressive nature of this tumor is consistent with high uniform expression of epidermal growth factor receptor (EGFR) and a functionally significant proportion of ALDH(+) CSCs.

MeSH Terms
Animals Biopsy, Needle Carcinoma, Squamous Cell/genetics,pathology Cell Line, Tumor Gene Expression Regulation, Neoplastic Genes, p53/genetics Genotype Head and Neck Neoplasms/genetics,pathology Human papillomavirus 16/genetics,isolation & purification Humans Immunohistochemistry In Situ Hybridization Mice Neoplastic Stem Cells/metabolism,pathology Papillomavirus Infections/genetics,pathology RNA, Viral/metabolism Reverse Transcriptase Polymerase Chain Reaction/methods Sensitivity and Specificity Squamous Cell Carcinoma of Head and Neck Transplantation, Heterologous
Chemicals
RNA, Viral
Authors & Affiliations
14 authors, click to expand affiliations / ORCID
Tang Alice L
Department of Otolaryngology-Head & Neck Surgery, University of Michigan, Ann Arbor, Michigan, USA.
Hauff Samantha J
Owen John H
Graham Martin P
Czerwinski Michael J
Park Jung Je
Walline Heather
Papagerakis Silvana
Stoerker Jay
McHugh Jonathan B
Chepeha Douglas B
Bradford Carol R
Carey Thomas E
Prince Mark E
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Article Info
Journal
Head & neck
Abbr.
Head Neck
ISSN
1097-0347
DOI
10.1002/hed.21962
Published
2012-10-00
Epub
2011-00-13
Pages
1480-91
Language
English
Region
United States
NLM ID
8902541
PMCID
PMC3369005
Subset
IM
Grants
NCI NIH HHS · P50 CA97248 · United States
NCI NIH HHS · P30 CA046592-23 · United States
NIDCD NIH HHS · T32 DC005356-04 · United States
NCI NIH HHS · P30 CA046592 · United States
NIDCR NIH HHS · R01 DE019126-04 · United States
NIDCD NIH HHS · P30 DC005188 · United States
NIDCD NIH HHS · T32 DC005356 · United States
NIDCR NIH HHS · 1 R01-DE019126 · United States
NCI NIH HHS · P50 CA097248-10 · United States
NIDCD NIH HHS · P30 DC005188-10 · United States
NIDCD NIH HHS · P30 DC05188 · United States
NCI NIH HHS · P50 CA097248 · United States
NIDCR NIH HHS · R01 DE019126 · United States
NIDCD NIH HHS · 5 T32 DC005356 · United States
NCI NIH HHS · P30 CA46592 · United States
External Links
PubMed source DOI: 10.1002/hed.21962 PMC Full Text View journal details
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