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PMID: 22138822 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

Controlled gene expression in primary Lgr5 organoid cultures.

Nature methods ·Vol. 9 ·No. 1 ·2011-12-04 ·Pages 81-3

Koo BK, Stange DE, Sato T, Karthaus W, Farin HF, Huch M, van Es JH, Clevers H

Abstract

The study of gene function in endodermal epithelia such as of stomach, small intestine and colon relies heavily on transgenic approaches. Establishing such animal models is laborious, expensive and time-consuming. We present here a method based on Cre recombinase-inducible retrovirus vectors that allows the conditional manipulation of gene expression in primary mouse organoid culture systems.

MeSH Terms

Animals Gene Expression Gene Knockdown Techniques/methods Green Fluorescent Proteins/genetics Humans Integrases/metabolism Intestinal Mucosa/cytology Mice Organoids/cytology,metabolism Receptors, G-Protein-Coupled/physiology Receptors, Notch/physiology Retroviridae/genetics Stem Cells/virology Tamoxifen/analogs & derivatives,pharmacology Transduction, Genetic/methods

Chemicals

Lgr5 protein, mouse Receptors, G-Protein-Coupled Receptors, Notch enhanced green fluorescent protein Tamoxifen Green Fluorescent Proteins afimoxifene Cre recombinase Integrases

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Koo Bon-Kyoung

Hubrecht Institute for Developmental Biology and Stem Cell Research, and University Medical Centre, Utrecht, The Netherlands.

Stange Daniel E

Sato Toshiro

Karthaus Wouter

Farin Henner F

Huch Meritxell

van Es Johan H

Clevers Hans

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14 references, click to expand

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Article Info

Journal

Nature methods

Abbr.

Nat Methods

ISSN

1548-7105

DOI

10.1038/nmeth.1802

Published

2011-12-04

Epub

2011-00-04

Pages

81-3

Language

English

Region

United States

NLM ID

101215604

Subset

Grants

Wellcome Trust · 104151 · United Kingdom

External Links

PubMed source DOI: 10.1038/nmeth.1802 View journal details

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