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PMID: 2212954 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Activation of platelets by eosinophil granule proteins.

The Journal of experimental medicine ·Vol. 172 ·No. 4 ·1990-10-01 ·Pages 1271-4

Rohrbach MS, Wheatley CL, Slifman NR, Gleich GJ

Abstract

Two of the four principal cationic proteins of the eosinophil granule, major basic protein (MBP) and eosinophil peroxidase (EPO), were shown to be platelet agonists. Both MBP and EPO evoked a dose-dependent nonlytic secretion of platelet 5-hydroxytryptamine in unstirred platelet suspensions even in the presence of 10 microM indomethacin. MBP also evoked secretion of platelet alpha granule and lysosome components. Secretion by MBP and EPO was inhibited by 1 microM PGE1, but the nature of the inhibition differed from that observed with thrombin. Thus, MBP and EPO can be classified as strong platelet agonists with a distinct mechanism of activation.

MeSH Terms

Alprostadil/pharmacology Blood Proteins/pharmacology Eosinophil Granule Proteins Eosinophils/enzymology,physiology Humans In Vitro Techniques Peroxidases/pharmacology Platelet Activation/drug effects Ribonucleases Serotonin/metabolism Thrombosis/etiology Thromboxane A2/biosynthesis

Chemicals

Blood Proteins Eosinophil Granule Proteins Serotonin Thromboxane A2 Peroxidases Ribonucleases Alprostadil

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Rohrbach M S

Department of Medicine, Mayo Clinic, Rochester, Minnesota 55905.

Wheatley C L

Slifman N R

Gleich G J

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26 references, click to expand

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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.172.4.1271

Published

1990-10-01

Pages

1271-4

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2188607

Subset

Grants

NIAID NIH HHS · AI-07047 · United States

NIAID NIH HHS · AI-09728 · United States

NHLBI NIH HHS · HL-28669 · United States

External Links

PubMed source DOI: 10.1084/jem.172.4.1271 PMC Full Text View journal details

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