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PMID: 22102820 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar.

PLoS pathogens ·Vol. 7 ·No. 11 ·2011-11-00 ·Pages e1002386

Felisberto-Rodrigues C, Durand E, Aschtgen MS, Blangy S, Ortiz-Lombardia M, Douzi B, Cambillau C, Cascales E

Abstract

Type VI secretion systems (T6SS) are trans-envelope machines dedicated to the secretion of virulence factors into eukaryotic or prokaryotic cells, therefore required for pathogenesis and/or for competition towards neighboring bacteria. The T6SS apparatus resembles the injection device of bacteriophage T4, and is anchored to the cell envelope through a membrane complex. This membrane complex is composed of the TssL, TssM and TagL inner membrane anchored proteins and of the TssJ outer membrane lipoprotein. Here, we report the crystal structure of the enteroaggregative Escherichia coli Sci1 TssJ lipoprotein, a two four-stranded β-sheets protein that exhibits a transthyretin fold with an additional α-helical domain and a protruding loop. We showed that TssJ contacts TssM through this loop since a loop depleted mutant failed to interact with TssM in vitro or in vivo. Biophysical analysis of TssM and TssJ-TssM interaction suggest a structural model of the membrane-anchored outer shell of T6SS. Collectively, our results provide an improved understanding of T6SS assembly and encourage structure-aided drug design of novel antimicrobials targeting T6SS.

MeSH Terms
Amino Acid Sequence Bacterial Outer Membrane Proteins/chemistry,metabolism Bacterial Secretion Systems Cell Membrane/metabolism Escherichia coli/metabolism,pathogenicity Escherichia coli Proteins/chemistry,metabolism Lipopeptides/chemistry,metabolism Membrane Proteins/chemistry,metabolism Protein Folding Protein Structure, Tertiary Sequence Alignment Virulence Factors/chemistry,metabolism
Chemicals
Bacterial Outer Membrane Proteins Bacterial Secretion Systems Escherichia coli Proteins Lipopeptides Membrane Proteins TssJ protein, E coli TssL protein, E coli Virulence Factors inner membrane protein, E coli
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Felisberto-Rodrigues Catarina
Aix-Marseille Université, Architecture et Fonction des Macromolécules Biologiques, Campus de Luminy, Marseille, France.
Durand Eric
Aschtgen Marie-Stéphanie
Blangy Stéphanie
Ortiz-Lombardia Miguel
Douzi Badreddine
Cambillau Christian
Cascales Eric
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Article Info
Journal
PLoS pathogens
Abbr.
PLoS Pathog
ISSN
1553-7374
DOI
10.1371/journal.ppat.1002386
Published
2011-11-00
Epub
2011-00-10
Pages
e1002386
Language
English
Region
United States
NLM ID
101238921
PMCID
PMC3213119
Subset
IM
Databases
PDB
3RX9
External Links
PubMed source DOI: 10.1371/journal.ppat.1002386 PMC Full Text View journal details
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