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PMID: 22080429 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Phyllosphere bacterial communities of trichome-bearing and trichomeless Arabidopsis thaliana leaves.

Antonie van Leeuwenhoek ·Vol. 101 ·No. 3 ·2012-03-00 ·Pages 551-60

Reisberg EE, Hildebrandt U, Riederer M, Hentschel U

Abstract

This study aimed to investigate whether the presence of trichomes as conspicuous physical attributes of the leaf surface affects the microbial community composition on Arabidopsis thaliana leaves. The A. thaliana ecotype Col-0 and its trichomeless gl1 mutant were grown in growth cabinets under climate-controlled conditions. The gl1 mutant showed a similar wax composition as the Col-0 wild type with slightly reduced amounts of C(29), C(31) and C(33) alkanes by GC/MS and GC/FID analyses. 120 bacterial isolates representing 39 bacterial genera were obtained from A. thaliana Col-0 leaf surfaces. Phylogenetic analysis of nearly full-length 16S rRNA sequences from 29 selected isolates confirmed their affiliation to the Proteobacteria (Alpha-, Beta-, Gamma-), Actinobacteria, Bacteroidetes and Firmicutes. The bacterial diversity on A. thaliana ecotype Col-0 and its gl1 mutant, devoid of trichomes, were further compared by denaturing gradient gel electrophoresis (DGGE). Banding patterns and sequencing of representative DGGE bands revealed the presence of phylotypes related to Sphingomonas (Alphaproteobacteria), Methylophilus (Betaproteobacteria) and Dyadobacter (Bacteroidetes) which are common phyllosphere inhabitants. Furthermore, wildtype and trichomeless mutant plants were exposed to outdoor conditions for 4-5 weeks. The DGGE gels showed only minor differences between the two plant lines, thus suggesting that trichomes per se do not affect bacterial diversity on Arabidopsis leaves under the experimental conditions tested.

MeSH Terms

Arabidopsis/genetics,microbiology Arabidopsis Proteins/genetics Bacteria/classification,genetics,isolation & purification,metabolism Bacterial Adhesion Bacterial Load DNA, Bacterial/genetics DNA, Ribosomal/genetics DNA-Binding Proteins/deficiency,genetics Metagenome/genetics Microbial Consortia Molecular Sequence Data Phylogeny Plant Leaves/chemistry,microbiology,ultrastructure RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Sequence Homology, Nucleic Acid Waxes/analysis

Chemicals

Arabidopsis Proteins DNA, Bacterial DNA, Ribosomal DNA-Binding Proteins RNA, Bacterial RNA, Ribosomal, 16S Waxes GL1 protein, Arabidopsis

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Reisberg Eva E

Julius-von-Sachs-Institut für Biowissenschaften, Universität Würzburg, Lehrstuhl für Botanik II, Würzburg, Germany.

Hildebrandt Ulrich

Riederer Markus

Hentschel Ute

Article Info

Journal

Antonie van Leeuwenhoek

Abbr.

Antonie Van Leeuwenhoek

ISSN

1572-9699

DOI

10.1007/s10482-011-9669-8

Published

2012-03-00

Epub

2011-00-13

Pages

551-60

Language

English

Region

Netherlands

NLM ID

0372625

Subset

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External Links

PubMed source DOI: 10.1007/s10482-011-9669-8 View journal details

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Phyllosphere bacterial communities of trichome-bearing and trichomeless Arabidopsis thaliana leaves.

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