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PMID: 2203302 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Inhibition of Pneumocystis carinii dihydropteroate synthetase by sulfa drugs.

Antimicrobial agents and chemotherapy ·Vol. 34 ·No. 6 ·1990-06-00 ·Pages 1075-8

Merali S, Zhang Y, Sloan D, Meshnick S

Abstract

A new reversed-phase high-pressure liquid chromatography assay procedure for dihydropteroate synthetase (DHPS) that involves the elution of the enzyme incubation solution with a series of three solvents of decreasing polarity (ammonium phosphate buffer, 10% methanol, and 50% methanol) was designed. By this procedure DHPS was detected in Escherichia coli and Pneumocystis carinii with specific activities of 450 and 14 U/mg, respectively. A comparison of the effects of five sulfa drugs on P. carinii DHPS activity revealed that dapsone is the most potent of these drugs.

MeSH Terms
Animals Chromatography, High Pressure Liquid Dihydropteroate Synthase/antagonists & inhibitors Escherichia coli/enzymology Fungal Proteins/isolation & purification Male Phosphorylation Pneumocystis/drug effects,enzymology Pterins/chemical synthesis Rats Rats, Inbred Strains Sulfonamides/pharmacology Sulfones/pharmacology Transferases/antagonists & inhibitors
Chemicals
Fungal Proteins Pterins Sulfonamides Sulfones Transferases Dihydropteroate Synthase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Merali S
Department of Chemistry, City University of New York Medical School, City College of City University of New York, New York 10031.
Zhang Y
Sloan D
Meshnick S
References (13)
13 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.34.6.1075
Published
1990-06-00
Pages
1075-8
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC171761
Subset
IM
Grants
NCRR NIH HHS · RR-01868 · United States
NCRR NIH HHS · RR-03060 · United States
External Links
PubMed source DOI: 10.1128/AAC.34.6.1075 PMC Full Text View journal details
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