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PMID: 2201751 Published · ppublish English Comparative Study Journal Article

Cloning and characterization of cDNAs for murine macrophage inflammatory protein 2 and its human homologues.

The Journal of experimental medicine ·Vol. 172 ·No. 3 ·1990-09-01 ·Pages 911-9

Tekamp-Olson P, Gallegos C, Bauer D, McClain J, Sherry B, Fabre M, van Deventer S, Cerami A

Abstract

A cDNA clone of murine macrophage inflammatory protein 2 (MIP-2) has been isolated from a library prepared from lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and the nucleotide sequence determined. This cDNA was used to clone cDNAs for human homologues of MIP-2 from a library prepared from phorbol myristate acetate-treated and LPS-stimulated U937 cells. Two homologues were isolated and sequenced. Human MIP-2 alpha and MIP-2 beta are highly homologous to each other and to a previously isolated gene, human gro/melanoma growth-stimulating activity (MGSA). These three human genes, MIP-2 alpha, MIP-2 beta, and gro/MGSA, constitute a sub-family within the cytokine family represented by platelet factor 4 and interleukin 8.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Carrier Proteins/genetics Cell Line Chemokine CXCL2 Chemokines, CXC Chemotactic Factors/genetics Cloning, Molecular DNA/genetics,isolation & purification Gene Library Humans Intercellular Signaling Peptides and Proteins Interleukin-8 Mice Molecular Sequence Data Oligonucleotide Probes Sequence Homology, Nucleic Acid
Chemicals
CXCL2 protein, human CXCL3 protein, human Carrier Proteins Chemokine CXCL2 Chemokines, CXC Chemotactic Factors Intercellular Signaling Peptides and Proteins Interleukin-8 Oligonucleotide Probes DNA
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Tekamp-Olson P
Chiron Corporation, Emeryville, California 94608.
Gallegos C
Bauer D
McClain J
Sherry B
Fabre M
van Deventer S
Cerami A
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.172.3.911
Published
1990-09-01
Pages
911-9
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188540
Subset
IM
Databases
GENBANK
X53798, X53799, X53800
External Links
PubMed source DOI: 10.1084/jem.172.3.911 PMC Full Text View journal details
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