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PMID: 22000032 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Diversity and biogeography of selected phyllosphere bacteria with special emphasis on Methylobacterium spp.

Systematic and applied microbiology ·Vol. 34 ·No. 8 ·2011-12-00 ·Pages 621-30

Wellner S, Lodders N, Kämpfer P

Abstract

On the basis of cultivation-dependent (isolation on mineral salt medium supplemented with 0.5% methanol) and -independent (DGGE analysis) methods, we investigated the influence of the host plant species Trifolium repens and Cerastium holosteoides, three geographic locations and the land-use types meadow, mown pasture and pasture on the abundance and community composition of selected phyllosphere bacteria with emphasis on Methylobacterium species. Methylobacterium abundance was significantly higher on leaves of T. repens (mean value 2.0×10(7) CFU PPFM per g leaf) than on leaves of C. holosteoides (mean value 2.0×10(6) CFU per g leaf). Leaves from the sampling site Schorfheide-Chorin showed slightly lower Methylobacterium numbers than leaves of the other sampling sites. Land-use and sampling period had no consistent influence on Methylobacterium community size. Methylobacterium community composition was very similar over both sampling periods, all three sampling sites, all land-use types and both plant species. Moreover, no relationship between geographic and genetic distance was observed. Community composition of selected Proteobacteria was influenced by plant species, geographic location and land-use. Often, differences in community composition could be observed between meadows, mown pastures and pastures but not between different kinds of meadows (cutted once versus three times) and mown pastures (fertilized versus non-fertilized). The results also indicate, that whether there are differences between land-use types or not strongly depends on the investigated host plant species and ecosystem. Besides Methylobacterium, representatives of Methylophilus were detected. The results indicate that Methylobacterium species are generally abundant and stable members of the phyllosphere community whereas other genera occur more occasionally, and that Methylobacterium clearly dominates the methylotrophic phyllosphere community.

MeSH Terms

Bacteria/classification,genetics Culture Media Ecosystem Electrophoresis Germany Methylobacterium/classification,genetics,isolation & purification Molecular Sequence Data Plant Leaves/microbiology Plants/microbiology Sequence Analysis, DNA Soil Microbiology Species Specificity Trifolium/microbiology

Chemicals

Culture Media

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Wellner S

Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.

Lodders N

Kämpfer P

Article Info

Journal

Systematic and applied microbiology

Abbr.

Syst Appl Microbiol

ISSN

1618-0984

DOI

10.1016/j.syapm.2011.08.005

Published

2011-12-00

Epub

2011-00-13

Pages

621-30

Language

English

Region

Germany

NLM ID

8306133

Subset

Databases

GENBANK

FR733885, FR733886, FR847846, FR847847, FR847848, FR869796, FR869797, FR869798, FR869799, FR869800, FR869801, FR869802, FR869803, FR869804, FR869805, FR869806, FR869807, FR869808, FR869809, FR869810, FR869811, FR869812, FR869813, FR869814, FR869815, FR869816, FR869817, FR869818, FR869819, FR869820, FR869821, FR869822, FR869823, FR869824, FR869825, FR869826, FR869827, FR869828, FR869829, FR869830, FR869831, FR869832, FR869833, FR869834, FR869835

External Links

PubMed source DOI: 10.1016/j.syapm.2011.08.005 View journal details

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Diversity and biogeography of selected phyllosphere bacteria with special emphasis on Methylobacterium spp.

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