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PMID: 21998657 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

MMP-10/stromelysin-2 promotes invasion of head and neck cancer.

PloS one ·Vol. 6 ·No. 10 ·2011-00-00 ·Pages e25438

Deraz EM, Kudo Y, Yoshida M, Obayashi M, Tsunematsu T, Tani H, Siriwardena SB, Keikhaee MR, Kiekhaee MR, Qi G, Iizuka S, Ogawa I, Campisi G, Lo Muzio L, Abiko Y, Kikuchi A, Takata T

Abstract

Periostin, IFN-induced transmembrane protein 1 (IFITM1) and Wingless-type MMTV integration site family, member 5B (Wnt-5b) were previously identified as the invasion promoted genes of head and neck squamous cell carcinoma (HNSCC) by comparing the gene expression profiles between parent and a highly invasive clone. We have previously reported that Periostin and IFITM1 promoted the invasion of HNSCC cells. Here we demonstrated that Wnt-5b overexpression promoted the invasion of HNSCC cells. Moreover, stromelysin-2 (matrix metalloproteinase-10; MMP-10) was identified as a common up-regulated gene among Periostin, IFITM1 and Wnt-5b overexpressing HNSCC cells by using microarray data sets. In this study, we investigated the roles of MMP-10 in the invasion of HNSCC. We examined the expression of MMP-10 in HNSCC cases by immunohistochemistry. High expression of MMP-10 was frequently observed and was significantly correlated with the invasiveness and metastasis in HNSCC cases. Next, we examined the roles of MMP-10 in the invasion of HNSCC cells in vitro. Ectopic overexpression of MMP-10 promoted the invasion of HNSCC cells, and knockdown of MMP-10 suppressed the invasion of HNSCC cells. Moreover, MMP-10 knockdown suppressed Periostin and Wnt-5b-promoted invasion. Interestingly, MMP-10 overexpression induced the decreased p38 activity and MMP-10 knockdown induced the increased p38 activity. In addition, treatment with a p38 inhibitor SB203580 in HNSCC cells inhibited the invasion. These results suggest that MMP-10 plays an important role in the invasion and metastasis of HNSCC, and that invasion driven by MMP-10 is partially associated with p38 MAPK inhibition. We suggest that MMP-10 can be used as a marker for prediction of metastasis in HNSCC.

MeSH Terms
Antigens, Differentiation/genetics Carcinoma, Squamous Cell/genetics,metabolism,pathology Cell Adhesion Molecules/genetics Cell Line, Tumor Extracellular Matrix/drug effects,metabolism Gene Expression Regulation, Neoplastic/drug effects Gene Knockdown Techniques Head and Neck Neoplasms/genetics,metabolism,pathology Humans Matrix Metalloproteinase 10/deficiency,genetics,metabolism Neoplasm Invasiveness Neoplasm Metastasis Protein Kinase Inhibitors/pharmacology Up-Regulation/drug effects Wnt Proteins/genetics p38 Mitogen-Activated Protein Kinases/antagonists & inhibitors
Chemicals
Antigens, Differentiation Cell Adhesion Molecules POSTN protein, human Protein Kinase Inhibitors WNT5B protein, human Wnt Proteins leu-13 antigen p38 Mitogen-Activated Protein Kinases Matrix Metalloproteinase 10
Authors & Affiliations
17 authors, click to expand affiliations / ORCID
Deraz Elsayed Mohamed
Division of Frontier Medical Science, Department of Oral and Maxillofacial Pathobiology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
Kudo Yasusei
Yoshida Maki
Obayashi Mariko
Tsunematsu Takaaki
Tani Hirotaka
Siriwardena Samadarani B S M
Keikhaee Mohammad Reza
Kiekhaee Mohammad Reza
Qi Guangying
Iizuka Shinji
Ogawa Ikuko
Campisi Giuseppina
Lo Muzio Lorenzo
Abiko Yoshimitsu
Kikuchi Akira
Takata Takashi
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0025438
Published
2011-00-00
Epub
2011-00-05
Pages
e25438
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC3187776
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1371/journal.pone.0025438 PMC Full Text View journal details
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