Abstract
Insulators are multiprotein-DNA complexes thought to affect gene expression by mediating inter- and intrachromosomal interactions. Drosophila insulators contain specific DNA-binding proteins plus common components, such as CP190, that facilitate these interactions. Here, we examine changes in the distribution of Drosophila insulator proteins during the heat-shock and ecdysone responses. We find that CP190 recruitment to insulator sites is the main regulatable step in controlling insulator function during heat shock. In contrast, both CP190 and DNA-binding protein recruitment are regulated during the ecdysone response. CP190 is necessary to stabilize specific chromatin loops and for proper activation of transcription of genes regulated by this hormone. These findings suggest that cells may regulate recruitment of insulator proteins to DNA to activate insulator activity at specific sites and create distinct patterns of nuclear organization that are necessary to achieve proper gene expression in response to different stimuli.
MeSH Terms
Animals
Chromatin/metabolism
Chromosomes/chemistry
DNA/chemistry
DNA-Binding Proteins/metabolism
Drosophila
Drosophila Proteins/metabolism
Ecdysone/pharmacology
Gene Expression Profiling
Gene Expression Regulation, Developmental
Genome
Heat-Shock Proteins/metabolism
Microscopy, Fluorescence/methods
Microtubule-Associated Proteins/metabolism
Nuclear Proteins/metabolism
Protein Binding
Transcription, Genetic
Chemicals
CP190 protein, Drosophila
Chromatin
DNA-Binding Proteins
Drosophila Proteins
Heat-Shock Proteins
Microtubule-Associated Proteins
Nuclear Proteins
Ecdysone
DNA
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Wood Ashley M
Department of Biology, Emory University, Atlanta, GA 30322, USA.
Van Bortle Kevin
Ramos Edward
Takenaka Naomi
Rohrbaugh Margaret
Jones Brian C
Jones Keith C
Corces Victor G
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