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PMID: 21949069 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Tropodithietic acid production in Phaeobacter gallaeciensis is regulated by N-acyl homoserine lactone-mediated quorum sensing.

Journal of bacteriology ·Vol. 193 ·No. 23 ·2011-12-00 ·Pages 6576-85

Berger M, Neumann A, Schulz S, Simon M, Brinkhoff T

Abstract

The production of N-acyl homoserine lactones (AHLs) is widely distributed within the marine Roseobacter clade, and it was proposed that AHL-mediated quorum sensing (QS) is one of the most common cell-to-cell communication mechanisms in roseobacters. The traits regulated by AHL-mediated QS are yet not known for members of the Roseobacter clade, but production of the antibiotic tropodithietic acid (TDA) was supposed to be controlled by AHL-mediated QS in Phaeobacter spp. We describe here for the first time the functional role of luxR and luxI homologous genes of an organism of the Roseobacter clade, i.e., pgaR and pgaI in Phaeobacter gallaeciensis. Our results demonstrate that the AHL synthase gene pgaI is responsible for production of N-3-hydroxydecanoylhomoserine lactone (3OHC(10)-HSL). Insertion mutants of pgaI and pgaR are both deficient in TDA biosynthesis and the formation of a yellow-brown pigment when grown in liquid marine broth medium. This indicates that in P. gallaeciensis the production of both secondary metabolites is controlled by AHL-mediated QS. Quantitative real-time PCR showed that the transcription level of tdaA, which encodes an essential transcriptional regulator for TDA biosynthesis, decreased 28- and 51-fold in pgaI and pgaR genetic backgrounds, respectively. These results suggest that both the response regulator PgaR and the 3OHC(10)-HSL produced by PgaI induce expression of tdaA, which in turn positively regulates expression of the tda genes. Moreover, we confirmed that TDA can also act as autoinducer in P. gallaeciensis, as previously described for Silicibacter sp. strain TM1040, but only in the presence of the response regulator PgaR.

MeSH Terms
Acyl-Butyrolactones/metabolism Bacterial Proteins/genetics,metabolism Gene Expression Regulation, Bacterial Quorum Sensing Roseobacter/genetics,physiology Tropolone/analogs & derivatives,metabolism
Chemicals
Acyl-Butyrolactones Bacterial Proteins tropodithietic acid Tropolone
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Berger Martine
Institute for Chemistry and Biology of the Marine Environment, University of Oldenburg, D-26111 Oldenburg, Germany.
Neumann Alexander
Schulz Stefan
Simon Meinhard
Brinkhoff Thorsten
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
1098-5530
DOI
10.1128/JB.05818-11
Published
2011-12-00
Epub
2011-00-23
Pages
6576-85
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC3232910
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.05818-11 PMC Full Text View journal details
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