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PMID: 2193912 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of ompF domains involved in Escherichia coli K-12 sensitivity to colicins A and N.

Journal of bacteriology ·Vol. 172 ·No. 7 ·1990-07-00 ·Pages 3675-80

Fourel D, Hikita C, Bolla JM, Mizushima S, Pagès JM

Abstract

Various ompF-ompC, ompC-ompF, and ompF-ompC-ompF chimeric genes were used to locate the domains of the OmpF protein involved in cellular sensitivity to colicins. Various parts of the porin participate in the entry of colicins. Colicin N receptor activity was found to require three regions: RN1, located between residues 1 and 63; RN2, located between residues 115 and 262; and RN3, located between residues 279 and 297. The central domain from residues 143 to 262 is involved during the translocation step after the binding step. A large region, including residues 1 to 262, is necessary during colicin A entry. The locations and interactions between these domains specifically required for the uptake of colicins to occur are described and discussed with regard to the homology and topology of the OmpC, OmpF, and PhoE porins.

MeSH Terms
Amino Acid Sequence Bacterial Outer Membrane Proteins/genetics Binding Sites Chimera Colicins/metabolism,pharmacology Escherichia coli/drug effects,genetics,growth & development Genes, Bacterial Molecular Sequence Data
Chemicals
Bacterial Outer Membrane Proteins Colicins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Fourel D
Centre de Biochimie et de Biologie Moléculaire, Centre National de la Recherche Scientifique, Marseille, France.
Hikita C
Bolla J M
Mizushima S
Pagès J M
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33 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.7.3675-3680.1990
Published
1990-07-00
Pages
3675-80
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213342
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.172.7.3675-3680.1990 PMC Full Text View journal details
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