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题目:: Human ovarian tumor cells escape γδ T cell recognition partly by down regulating surface expression of MICA and limiting cell cycle related molecules.
作者:: Lu(Jingwei),Aggarwal(Reeva),Kanji(Suman),Das(Manjusri),Joseph(Matthew),Pompili(Vincent),Das(Hiranmoy)
状态:: 发布时间2011-09-21 , 更新时间 2016-10-19
期刊:: PLoS One
摘要:: Mechanisms of human Vγ2Vδ2 T cell-mediated tumor immunity have yet to be fully elucidated.,At least some tumor cell recognition is mediated by NKG2D-MICA interactions. Herein, by using MTT assay and PI-BrdU co-staining and Western-blot, we show that these Vγ2Vδ2 T cells can limit the proliferation of ovarian tumor cells by down regulation of apoptosis and cell cycle related molecules in tumor cells. Cell-to-cell contact is critical. γδ T cell-resistant, but not susceptible ovarian tumor cells escape γδ T cell-mediated immune recognition by up-regulating pErk1/2, thereby decreasing surface MICA levels. Erk1/2 inhibitor pretreatment or incubation prevents this MICA decrease, while up-regulating key cell cycle related molecules such as CDK2, CDK4 and Cyclin D1, as well as apoptosis related molecules making resistant tumor cells now vulnerable to γδ T cell-mediated lysis.,These findings demonstrate novel effects of γδT cells on ovarian tumor cells.
语言:: eng
DOI:

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