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PMID: 2190218 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Glucose transporters are abundant in cells with "occluding" junctions at the blood-eye barriers.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 11 ·1990-06-00 ·Pages 4261-4

Harik SI, Kalaria RN, Whitney PM, Andersson L, Lundahl P, Ledbetter SR, Perry G

Abstract

We studied the distribution of the "erythroid/brain" glucose transporter protein in the human and rat eye by immunocytochemistry with monoclonal and polyclonal antibodies to the C terminus of the human erythrocyte glucose transporter. We found intense immunocytochemical staining in the endothelium of microvessels of the retina, optic nerve, and iris but not in microvessels of the choroid, ciliary body, sclera, and other retro-orbital tissues. In addition, we found marked immunocytochemical staining of retinal pigment epithelium, ciliary body epithelium, and posterior epithelium of the iris. The common feature of all those endothelial and epithelial cells that stained intensely for the glucose transporter is the presence of "occluding" intercellular junctions, which constitute the anatomical bases of the blood-eye barriers. We propose that a high density of the glucose transporter is a biochemical concomitant of epithelial and endothelial cells with barrier characteristics, at least in tissues that have a high metabolic requirement for glucose.

MeSH Terms
Animals Endothelium, Vascular/cytology,metabolism Epithelium/metabolism,ultrastructure Eye/blood supply,metabolism Glucose/metabolism Humans Immunoenzyme Techniques Intercellular Junctions/ultrastructure Microcirculation/metabolism Monosaccharide Transport Proteins/metabolism Rats Rats, Inbred Strains
Chemicals
Monosaccharide Transport Proteins Glucose
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Harik S I
Department of Neurology, University Hospitals of Cleveland, OH 44106.
Kalaria R N
Whitney P M
Andersson L
Lundahl P
Ledbetter S R
Perry G
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.11.4261
Published
1990-06-00
Pages
4261-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54088
Subset
IM
Grants
NIA NIH HHS · AG-00415 · United States
NHLBI NIH HHS · HL-35617 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.11.4261 PMC Full Text View journal details
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