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PMID: 21866101 Published · epublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Small molecule inhibitors reveal Niemann-Pick C1 is essential for Ebola virus infection.

Nature ·Vol. 477 ·No. 7364 ·2011-08-24 ·Pages 344-8

Côté M, Misasi J, Ren T, Bruchez A, Lee K, Filone CM, Hensley L, Li Q, Ory D, Chandran K, Cunningham J

Abstract

Ebola virus (EboV) is a highly pathogenic enveloped virus that causes outbreaks of zoonotic infection in Africa. The clinical symptoms are manifestations of the massive production of pro-inflammatory cytokines in response to infection and in many outbreaks, mortality exceeds 75%. The unpredictable onset, ease of transmission, rapid progression of disease, high mortality and lack of effective vaccine or therapy have created a high level of public concern about EboV. Here we report the identification of a novel benzylpiperazine adamantane diamide-derived compound that inhibits EboV infection. Using mutant cell lines and informative derivatives of the lead compound, we show that the target of the inhibitor is the endosomal membrane protein Niemann-Pick C1 (NPC1). We find that NPC1 is essential for infection, that it binds to the virus glycoprotein (GP), and that antiviral compounds interfere with GP binding to NPC1. Combined with the results of previous studies of GP structure and function, our findings support a model of EboV infection in which cleavage of the GP1 subunit by endosomal cathepsin proteases removes heavily glycosylated domains to expose the amino-terminal domain, which is a ligand for NPC1 and regulates membrane fusion by the GP2 subunit. Thus, NPC1 is essential for EboV entry and a target for antiviral therapy.

MeSH Terms
Adamantane/analogs & derivatives,chemistry Animals Antiviral Agents/chemistry,pharmacology Carrier Proteins/metabolism Cathepsins/metabolism Cell Line Chlorocebus aethiops Ebolavirus/drug effects,physiology Endosomes/enzymology Glycoproteins/metabolism Hemorrhagic Fever, Ebola/drug therapy,metabolism Humans Intracellular Signaling Peptides and Proteins Membrane Fusion/drug effects Membrane Glycoproteins/metabolism Molecular Weight Niemann-Pick C1 Protein Piperazines/chemistry Vero Cells Viral Fusion Proteins/metabolism Virus Internalization/drug effects
Chemicals
Antiviral Agents Carrier Proteins Glycoproteins Intracellular Signaling Peptides and Proteins Membrane Glycoproteins NPC1 protein, human Niemann-Pick C1 Protein Piperazines Viral Fusion Proteins Cathepsins Adamantane
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Côté Marceline
Division of Hematology, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA.
Misasi John
Ren Tao
Bruchez Anna
Lee Kyungae
Filone Claire Marie
Hensley Lisa
Li Qi
Ory Daniel
Chandran Kartik
Cunningham James
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Article Info
Journal
Nature
Abbr.
Nature
ISSN
1476-4687
Published
2011-08-24
Epub
2011-00-24
Pages
344-8
Language
English
Region
England
NLM ID
0410462
PMCID
PMC3230319
Subset
IM
Grants
NIAID NIH HHS · U54 AI057159-09 · United States
NIAID NIH HHS · U54 AI057159 · United States
NICHD NIH HHS · K12-HD052896 · United States
NIAID NIH HHS · R01 AI088027 · United States
NHLBI NIH HHS · T32 HL007623 · United States
NIAID NIH HHS · K08 AI079381 · United States
NHLBI NIH HHS · 5-T32-HL007623 · United States
NICHD NIH HHS · K12 HD052896 · United States
NCI NIH HHS · R01 CA104266 · United States
NIAID NIH HHS · 5K08AI079381 · United States
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