Abstract
Ebola virus (EboV) is a highly pathogenic enveloped virus that causes outbreaks of zoonotic infection in Africa. The clinical symptoms are manifestations of the massive production of pro-inflammatory cytokines in response to infection and in many outbreaks, mortality exceeds 75%. The unpredictable onset, ease of transmission, rapid progression of disease, high mortality and lack of effective vaccine or therapy have created a high level of public concern about EboV. Here we report the identification of a novel benzylpiperazine adamantane diamide-derived compound that inhibits EboV infection. Using mutant cell lines and informative derivatives of the lead compound, we show that the target of the inhibitor is the endosomal membrane protein Niemann-Pick C1 (NPC1). We find that NPC1 is essential for infection, that it binds to the virus glycoprotein (GP), and that antiviral compounds interfere with GP binding to NPC1. Combined with the results of previous studies of GP structure and function, our findings support a model of EboV infection in which cleavage of the GP1 subunit by endosomal cathepsin proteases removes heavily glycosylated domains to expose the amino-terminal domain, which is a ligand for NPC1 and regulates membrane fusion by the GP2 subunit. Thus, NPC1 is essential for EboV entry and a target for antiviral therapy.
MeSH Terms
Adamantane/analogs & derivatives,chemistry
Animals
Antiviral Agents/chemistry,pharmacology
Carrier Proteins/metabolism
Cathepsins/metabolism
Cell Line
Chlorocebus aethiops
Ebolavirus/drug effects,physiology
Endosomes/enzymology
Glycoproteins/metabolism
Hemorrhagic Fever, Ebola/drug therapy,metabolism
Humans
Intracellular Signaling Peptides and Proteins
Membrane Fusion/drug effects
Membrane Glycoproteins/metabolism
Molecular Weight
Niemann-Pick C1 Protein
Piperazines/chemistry
Vero Cells
Viral Fusion Proteins/metabolism
Virus Internalization/drug effects
Chemicals
Antiviral Agents
Carrier Proteins
Glycoproteins
Intracellular Signaling Peptides and Proteins
Membrane Glycoproteins
NPC1 protein, human
Niemann-Pick C1 Protein
Piperazines
Viral Fusion Proteins
Cathepsins
Adamantane
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Côté Marceline
Division of Hematology, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA.
Misasi John
Ren Tao
Bruchez Anna
Lee Kyungae
Filone Claire Marie
Hensley Lisa
Li Qi
Ory Daniel
Chandran Kartik
Cunningham James
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