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PMID: 2185743 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Thiol-dependent metallo-endopeptidase characteristics of Pz-peptidase in rat and rabbit.

The Biochemical journal ·Vol. 267 ·No. 2 ·1990-04-15 ·Pages 531-3

Tisljar U, Barrett AJ

Abstract

Pz-peptidase was purified from rat testis and rabbit muscle. Zinc was detectable in the rat enzyme. The activity of the enzyme from both species was slowly but completely abolished by EDTA and restored by Zn2+. Free thiol groups were also important for the catalytic activity of rat Pz-peptidase, as previously reported for the rabbit enzyme. We conclude that in both species Pz-peptidase has the characteristics of a thiol-dependent metallo-endopeptidase.

MeSH Terms

Amino Acid Sequence Animals Chromatography, DEAE-Cellulose Chromatography, Gel Chromatography, High Pressure Liquid Dithiothreitol/pharmacology Electrophoresis, Polyacrylamide Gel Endopeptidases/isolation & purification,metabolism Ethylmaleimide/pharmacology Kinetics Male Metalloendopeptidases/metabolism Molecular Sequence Data Molecular Weight Muscles/enzymology Rabbits Rats Substrate Specificity

Chemicals

Endopeptidases Metalloendopeptidases thimet oligopeptidase Ethylmaleimide Dithiothreitol

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Tisljar U

Department of Biochemistry, Strangeways Research Laboratory, Cambridge, U.K.

Barrett A J

References (11)

11 references, click to expand

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Purification and characterization of Pz-peptidase from rabbit muscle.
Arch Biochem Biophys. 1989 Oct;274(1):138-44 PMID: 2673041
Preparation, assay, and partial characterization of a neutral endopeptidase from rabbit brain.
Biochemistry. 1973 Apr 24;12(9):1838-44 PMID: 4699240
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The activities of 'Pz-peptidase' and 'endopeptidase 24.15' are due to a single enzyme.
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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2670531

Published

1990-04-15

Pages

531-3

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1131321

Subset

Grants

Wellcome Trust · United Kingdom

External Links

PubMed source DOI: 10.1042/bj2670531 PMC Full Text View journal details

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Thiol-dependent metallo-endopeptidase characteristics of Pz-peptidase in rat and rabbit.

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