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PMID: 21839040 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Bile acid is a host factor that regulates the composition of the cecal microbiota in rats.

Gastroenterology ·Vol. 141 ·No. 5 ·2011-11-00 ·Pages 1773-81

Islam KB, Fukiya S, Hagio M, Fujii N, Ishizuka S, Ooka T, Ogura Y, Hayashi T, Yokota A

Abstract

Alterations in the gastrointestinal microbiota have been associated with metabolic diseases. However, little is known about host factors that induce changes in gastrointestinal bacterial populations. We investigated the role of bile acids in this process because of their strong antimicrobial activities, specifically the effects of cholic acid administration on the composition of the gut microbiota in a rat model. Rats were fed diets supplemented with different concentrations of cholic acid for 10 days. We used 16S ribosomal RNA gene clone library sequencing and fluorescence in situ hybridization to characterize the composition of the cecal microbiota of the different diet groups. Bile acids in feces, organic acids in cecal contents, and some blood parameters were also analyzed. Administration of cholic acid induced phylum-level alterations in the composition of the gut microbiota; Firmicutes predominated at the expense of Bacteroidetes. Cholic acid feeding simplified the composition of the microbiota, with outgrowth of several bacteria in the classes Clostridia and Erysipelotrichi. Externally administered cholic acid was efficiently transformed into deoxycholic acid by a bacterial 7α-dehydroxylation reaction. Serum levels of adiponectin decreased significantly in rats given the cholic acid diet. Cholic acid regulates the composition of gut microbiota in rats, inducing similar changes to those induced by high-fat diets. These findings improve our understanding of the relationship between metabolic diseases and the composition of the gastrointestinal microbiota.

MeSH Terms

Adiponectin/blood Animals Bile Acids and Salts/analysis,metabolism Cecum/microbiology Cholic Acid/pharmacology Clostridium/drug effects,genetics,isolation & purification Dose-Response Relationship, Drug Erysipelothrix/drug effects,genetics,isolation & purification Feces/chemistry,microbiology Male Metagenome/drug effects,genetics,physiology Models, Animal Molecular Sequence Data RNA, Ribosomal, 16S Rats Rats, Inbred Strains

Chemicals

Adiponectin Bile Acids and Salts RNA, Ribosomal, 16S Cholic Acid

Authors & Affiliations

9 authors, click to expand affiliations / ORCID

Islam K B M Saiful

Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University, Sapporo, Japan.

Fukiya Satoru

Hagio Masahito

Fujii Nobuyuki

Ishizuka Satoshi

Ooka Tadasuke

Ogura Yoshitoshi

Hayashi Tetsuya

Yokota Atsushi

Article Info

Journal

Gastroenterology

Abbr.

Gastroenterology

ISSN

1528-0012

DOI

10.1053/j.gastro.2011.07.046

Published

2011-11-00

Epub

2011-00-10

Pages

1773-81

Language

English

Region

United States

NLM ID

0374630

Subset

Databases

GENBANK

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PubMed source DOI: 10.1053/j.gastro.2011.07.046 View journal details

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Bile acid is a host factor that regulates the composition of the cecal microbiota in rats.

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