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PMID: 2183623 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Neutrophil chemotactic factor (IL-8) gene expression by cytokine-treated retinal pigment epithelial cells.

The American journal of pathology ·Vol. 136 ·No. 4 ·1990-04-00 ·Pages 745-50

Elner VM, Strieter RM, Elner SG, Baggiolini M, Lindley I, Kunkel SL

Abstract

The neural-derived retinal pigment epithelium (RPE) underlies the sensory retina and is central to both retinal homeostasis and many common retinal diseases. Retinal pigment epithelium cells are actively phagocytic and share several features with macrophages that have recently been shown to produce a neutrophil chemotactic factor (NCF), also known as interleukin-8, after cytokine stimulation. Because RPE cell responses to cytokines are largely unknown, human RPE cell NCF production was monitored after interleukin-1-beta (IL-1 beta), tumor necrosis factor-alpha, or lipopolysaccharide stimulation. RPE NCF mRNA expression and RPE production of biologically active NCF was time and concentration dependent. Maximal NCF mRNA expression occurred at 20 ng/ml for IL-1 beta. Messenger RNA expression in RPE cells and biologically active NCF in RPE cell supernatants were found 1 hour after stimulation and were maintained for 24 hours. These findings demonstrate that cytokine-stimulated RPE cells may evoke or augment neutrophil-mediated inflammation by synthesizing NCF, a cytokine that may be important in ocular disease mechanisms.

MeSH Terms
Biological Assay Blotting, Northern Cells, Cultured Chemotactic Factors/genetics Chemotaxis Gene Expression/drug effects Humans In Vitro Techniques Interleukin-1/pharmacology Interleukin-8 Interleukins/genetics Lipopolysaccharides/pharmacology Pigment Epithelium of Eye/physiology RNA, Messenger/genetics Retina/physiology Tumor Necrosis Factor-alpha/pharmacology
Chemicals
Chemotactic Factors Interleukin-1 Interleukin-8 Interleukins Lipopolysaccharides RNA, Messenger Tumor Necrosis Factor-alpha
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Elner V M
Department of Opthalmology, (Kellogg Eye Center), University of Michigan, Ann Arbor.
Strieter R M
Elner S G
Baggiolini M
Lindley I
Kunkel S L
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1990-04-00
Pages
745-50
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1877641
Subset
IM
Grants
NIDDK NIH HHS · DK-38149 · United States
NHLBI NIH HHS · HL-31963 · United States
NHLBI NIH HHS · HL-35276 · United States
External Links
PubMed source PMC Full Text View journal details
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