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PMID: 2183197 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cloning and characterization of nuclear genes for two mitochondrial ribosomal proteins in Saccharomyces cerevisiae.

Nucleic acids research ·Vol. 18 ·No. 6 ·1990-03-25 ·Pages 1521-9

Kitakawa M, Grohmann L, Graack HR, Isono K

Abstract

The genes for two large subunit proteins, YmL8 and YmL20, of the mitochondrial ribosome of Saccharomyces cerevisiae were cloned by hybridization with synthetic oligonucleotide mixtures corresponding to their N-terminal amino acid sequences. They were termed MRP-L8 and MRP-L20, respectively, and their nucleotide sequences were determined using a DNA sequencer. The MRP-L8 gene was found to encode a 26.8-kDa protein whose deduced amino acid sequence has a high degree of similarity to ribosomal protein L17 of Escherichia coli. The gene MRP-L20 was found to encode a 22.3-kDa protein with a presequence consisting of 18 amino acid residues. By Southern blot hybridization to the yeast chromosomes separated by field-inversion gel electrophoresis, the MRP-L8 and MRP-L20 genes were located on chromosomes X and XI, respectively. Gene disruption experiments indicate that their products, YmL8 and YmL20 proteins, are essential for the mitochondrial function and the absence of these proteins causes instability of the mitochondrial DNA.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Nucleus/metabolism Chromosomes, Fungal Cloning, Molecular DNA, Mitochondrial/genetics Genes, Fungal Mitochondria/metabolism Molecular Sequence Data Restriction Mapping Ribosomal Proteins/genetics Saccharomyces cerevisiae/genetics,metabolism Sequence Homology, Nucleic Acid
Chemicals
DNA, Mitochondrial Ribosomal Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kitakawa M
Department of Biology, Faculty of Science, Kobe University, Japan.
Grohmann L
Graack H R
Isono K
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.6.1521
Published
1990-03-25
Pages
1521-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC330521
Subset
IM
Databases
GENBANK
X53840, X53841
External Links
PubMed source DOI: 10.1093/nar/18.6.1521 PMC Full Text View journal details
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