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PMID: 2183008 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The meta cleavage operon of TOL degradative plasmid pWW0 comprises 13 genes.

Molecular & general genetics : MGG ·Vol. 221 ·No. 1 ·1990-03-00 ·Pages 113-20

Harayama S, Rekik M

Abstract

The meta-cleavage operon of TOL plasmid pWW0 of Pseudomonas putida encodes a set of enzymes which transform benzoate/toluates to Krebs cycle intermediates via extradiol (meta-) cleavage of (methyl)catechol. The genetic organization of the operon was characterized by cloning of the meta-cleavage genes into an expression vector and identification of their products in Escherichia coli maxicells. This analysis showed that the meta-cleavage operon contains 13 genes whose order and products (in kilodaltons) are xylX(57)-xylY(20)-xylZ(39)-xylL(28)-xylT(1 2)-xylE(36)-xylG(60)-xylF(34)- xylJ(28)-xylQ(42)-xylK(39)-xylI(29)-xylH(4 ). The xylXYZ genes encode three subunits of toluate 1,2-dioxygenase. The xylL, xylE, xylG, xylF, xylJ, xylK, xylI, and xylH genes encode 1,2-dihydroxy-3,5-cyclohexadiene-1-carboxylate dehydrogenase, catechol 2,3-dioxygenase, 2-hydroxymuconic semialdehyde dehydrogenase, 2-hydroxymuconic semialdehyde hydrolase, 2-oxopent-4-enoate hydratase, 4-hydroxy-2-oxovalerate aldolase, 4-oxalocrotonate decarboxylase and 4-oxaloccotonate tautomerase, respectively. The functions of xylT and xylQ are not known at present. The comparison of the coding capacity and the sizes of the products of the meta-cleavage operon genes indicated that most of the DNA between xylX and xylH consists of coding sequences.

MeSH Terms
Bacterial Proteins/genetics Cloning, Molecular Electrophoresis, Polyacrylamide Gel Escherichia coli/genetics Genes, Bacterial Operon Plasmids Pseudomonas/enzymology,genetics Restriction Mapping
Chemicals
Bacterial Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Harayama S
Department of Medical Biochemistry, University of Geneva, Switzerland.
Rekik M
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35 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00280375
Published
1990-03-00
Pages
113-20
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00280375
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