Removal of cryptic poxvirus transcription termination signals from the human immunodeficiency virus type 1 envelope gene enhances expression and immunogenicity of a recombinant vaccinia virus.

Journal of virology ·Vol. 64 ·No. 5 ·1990-05-00 ·Pages 2448-51

Earl PL, Hügin AW, Moss B

Abstract

The in vivo role of the proposed poxvirus early transcription termination signal TTTTTNT was confirmed by analysis of the RNA species made by recombinant vaccinia viruses. Premature transcription termination occurred following each of two TTTTTNT sequences present naturally within the coding region of the human immunodeficiency virus type 1 envelope gene. Alteration of the TTTTTNT sequences, without changing the encoded amino acids, resulted in production of full-length early mRNAs, improved protein expression, and a more consistent immune response.

MeSH Terms

Animals Base Sequence Gene Products, env/analysis,genetics,isolation & purification Genes, Regulator Genes, env HIV Antibodies/immunology HIV Antigens/analysis HIV-1/genetics Mice Mice, Inbred C3H Molecular Sequence Data Mutation Oligonucleotide Probes Poxviridae/genetics RNA, Messenger/genetics Recombination, Genetic Terminator Regions, Genetic Transcription, Genetic Vaccinia virus/genetics

Chemicals

Gene Products, env HIV Antibodies HIV Antigens Oligonucleotide Probes RNA, Messenger

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Earl P L

Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.

Hügin A W

Moss B

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33 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.64.5.2448-2451.1990

Published

1990-05-00

Pages

2448-51

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC249414

Subset

External Links

PubMed source DOI: 10.1128/JVI.64.5.2448-2451.1990 PMC Full Text View journal details

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Removal of cryptic poxvirus transcription termination signals from the human immunodeficiency virus type 1 envelope gene enhances expression and immunogenicity of a recombinant vaccinia virus.

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