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PMID: 2178786 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Conditional hyporecombination mutants of three REC genes of Saccharomyces cerevisiae.

Current genetics ·Vol. 17 ·No. 1 ·1990-01-00 ·Pages 7-12

Esposito MS, Brown JT

Abstract

We have isolated and characterized three conditional hyporecombination mutants, rec1-1, rec3-1 and rec4-1, that define three REC genes of Saccharomyces cerevisiae required for spontaneous general mitotic interchromosomal recombination. Each MATa/MAT alpha rec/rec diploid is deficient in mitotic single site gene conversion, intragenic recombination, intergenic recombination and sporulation at the restrictive temperature (36 degrees C). The rec1-1 mutation also confers conditional enhanced sensitivity to the killing effects of X-rays. The rec1-1 and rec3-1 mutations have been mapped to chromosome VII. The rec1-1, rec3-1 and rec4-1 mutations exhibit complementation at 36 degrees C for both mitotic recombination and sporulation.

MeSH Terms
Chromosome Mapping Chromosomes, Fungal Diploidy Gene Conversion Genes, Fungal Genetic Complementation Test Mutation Phenotype Recombination, Genetic Saccharomyces cerevisiae/genetics,physiology Spores, Fungal Temperature
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Esposito M S
Cell and Molecular Biology Division, Lawrence Berkeley Laboratory, University of California, Berkeley 94720.
Brown J T
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00313242
Published
1990-01-00
Pages
7-12
Language
English
Region
United States
NLM ID
8004904
Subset
IM
Grants
NIGMS NIH HHS · GM 29002 · United States
External Links
PubMed source DOI: 10.1007/BF00313242 View journal details
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