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PMID: 21754999 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Role of SNX16 in the dynamics of tubulo-cisternal membrane domains of late endosomes.

PloS one ·Vol. 6 ·No. 7 ·2011-00-00 ·Pages e21771

Brankatschk B, Pons V, Parton RG, Gruenberg J

Abstract

In this paper, we report that the PX domain-containing protein SNX16, a member of the sorting nexin family, is associated with late endosome membranes. We find that SNX16 is selectively enriched on tubulo-cisternal elements of this membrane system, whose highly dynamic properties and formation depend on intact microtubules. By contrast, SNX16 was not found on vacuolar elements that typically contain LBPA, and thus presumably correspond to multivesicular endosomes. We conclude that SNX16, together with its partner phosphoinositide, define a highly dynamic subset of late endosomal membranes, supporting the notion that late endosomes are organized in distinct morphological and functional regions. Our data also indicate that SNX16 is involved in tubule formation and cholesterol transport as well as trafficking of the tetraspanin CD81, suggesting that the protein plays a role in the regulation of late endosome membrane dynamics.

MeSH Terms
Animals Antigens, CD/metabolism Brefeldin A/pharmacology Cell Line Cholesterol/metabolism Endosomes/drug effects,metabolism Humans Intracellular Membranes/drug effects,metabolism Membrane Microdomains/drug effects,metabolism Microscopy, Fluorescence Microtubules/drug effects,metabolism Polymerization/drug effects Protein Transport/drug effects Sorting Nexins/metabolism Subcellular Fractions/drug effects,metabolism Tetraspanin 28 Tissue Fixation
Chemicals
Antigens, CD CD81 protein, human Sorting Nexins Tetraspanin 28 Brefeldin A Cholesterol
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Brankatschk Ben
Department of Biochemistry, University of Geneva, Geneva, Switzerland.
Pons Véronique
Parton Robert G
Gruenberg Jean
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0021771
Published
2011-00-00
Epub
2011-00-06
Pages
e21771
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC3130770
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pone.0021771 PMC Full Text View journal details
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