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题目:: Laser-guidance-based cell deposition microscope for heterotypic single-cell micropatterning.
作者:: Ma(Zhen),Pirlo(Russell K),Wan(Qin),Yun(Julie X),Yuan(Xiaocong),Xiang(Peng),Borg(Thomas K),Gao(Bruce Z)
状态:: 发布时间2011-09-19 , 更新时间 2016-11-22
期刊:: Biofabrication
摘要:: Cell patterning methods enable researchers to control specific homotypic and heterotypic contact-mediated cell-cell and cell-ECM interactions and to impose defined cell and tissue geometries. To micropattern individual cells to specific points on a substrate with high spatial resolution, we have developed a cell deposition microscope based on the laser-guidance technique. We discuss the theory of optical forces for generating laser guidance and the optimization of the optical configuration (NA ≈ 0.1) to manipulate cells with high speed in three dimensions. Our cell deposition microscope is capable of patterning different cell types onto and within standard cell research devices and providing on-stage incubation for long-term cell culturing. Using this cell deposition microscope, rat mesenchymal stem cells from bone marrow were micropatterned with cardiomyocytes into a substrate microfabricated with polydimethylsiloxane on a 22 mm × 22 mm coverglass to form a single-cell coculturing microenvironment, and their electrophysiological property changes were investigated during the coculturing days.
语言:: eng
DOI:

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