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PMID: 2170339 Published · ppublish English Journal Article

Differential translation of cell division proteins.

Journal of bacteriology ·Vol. 172 ·No. 10 ·1990-10-00 ·Pages 6106-11

Mukherjee A, Donachie WD

Abstract

Cloned division genes (ftsQ and ftsA) and the gene for beta-lactamase (bla) were transcribed in vivo from a bacteriophage T7 promoter under conditions which blocked the use of other promoters. The different coding regions of single mRNAs were translated with widely different efficiencies, such that the ratio of beta-lactamase production to FtsQ production was about 75:1. The relative rates of translation of the division proteins reflected their relative rates of production from normal chromosomal promoters (FtsA greater than FtsQ). We show that the low rates of production of FtsQ and FtsA proteins are due to their ribosome-binding sequences and that there is no obligatory translational coupling between them, despite the close proximity of the genes. Levels of translation of FtsA are shown to be proportional to levels of transcription, and therefore there is no evidence of variable regulation of translation.

MeSH Terms
Bacterial Proteins/genetics Cell Division Chromosomes, Bacterial Cloning, Molecular Escherichia coli/cytology,enzymology,genetics Genes, Bacterial Plasmids Promoter Regions, Genetic Protein Biosynthesis RNA, Messenger/genetics T-Phages/genetics Transcription, Genetic beta-Lactamases/genetics
Chemicals
Bacterial Proteins RNA, Messenger beta-Lactamases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mukherjee A
Department of Molecular Biology, University of Edinburgh, Scotland.
Donachie W D
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20 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.10.6106-6111.1990
Published
1990-10-00
Pages
6106-11
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC526936
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.172.10.6106-6111.1990 PMC Full Text View journal details
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