Exposure of DNA bases induced by the interaction of DNA and calf thymus DNA helix-destabilizing protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 10 ·1978-10-00 ·Pages 4689-93

Kohwi-Shigematsu T, Enomoto T, Yamada MA, Nakanishi M, Tsuboi M

Abstract

The reaction of chloroacetaldehyde with adenine bases in DNA to give a fluorescent product was used to study the availability to intermolecular reaction of positions 1 and 6 of adenine in DNA complexes with calf thymus DNA helix-destabilizing protein. No inhibition of this reaction was observed when heat-denatured DNA was complexed with the protein at a protein/DNA weight ratio of 10:1, compared to free DNA. On the contrary, the same reaction was inhibited markedly for denatured DNA in the presence of calf thymus histone HI at protein/DNA weight ratio of 2:1. Furthermore, the exchange rate for hydrogens of amino and imide groups of DNA bases in DNA strands with deuterium in the solvent was totally unaffected upon complexing of DNA with the DNA helix-destabilizing protein as examined by stopped-flow ultraviolet spectroscopy. These results indicate that the DNA helix-destabilizing protein forms a complex with single-stranded DNA, leaving DNA bases uncovered by the protein. The fluorescence intensity of DNA pretreated with chloroacetaldehyde was amplified by nearly 3-fold upon addition of the DNA helix-destabilizing protein. The possibility of "unstacking" of DNA bases induced by the protein is discussed.

MeSH Terms

Acetaldehyde/analogs & derivatives,pharmacology Adenine/metabolism DNA/metabolism DNA Helicases/metabolism DNA, Single-Stranded/metabolism Histones/pharmacology Hot Temperature Hydrogen Nucleic Acid Conformation Nucleic Acid Denaturation

Chemicals

DNA, Single-Stranded Histones Hydrogen DNA DNA Helicases Acetaldehyde Adenine

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Kohwi-Shigematsu T

Enomoto T

Yamada M A

Nakanishi M

Tsuboi M

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21 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.75.10.4689

Published

1978-10-00

Pages

4689-93

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC336185

Subset

External Links

PubMed source DOI: 10.1073/pnas.75.10.4689 PMC Full Text View journal details

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Exposure of DNA bases induced by the interaction of DNA and calf thymus DNA helix-destabilizing protein.

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