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PMID: 2167506 Published · ppublish English Journal Article

Characterization of blood mononuclear cells producing IFN alpha following induction by coronavirus-infected cells (porcine transmissible gastroenteritis virus).

Research in immunology ·Vol. 141 ·No. 2 ·1990-02-00 ·Pages 141-51

Charley B, Lavenant L

Abstract

Porcine blood mononuclear cells (PBMC) were shown to produce interferon-alpha (IFN alpha) following incubation with cells infected by a coronavirus, transmissible gastroenteritis virus. Monoclonal antibodies (mAb) with specificities for leukocyte subsets and major histocompatibility complex (MHC) antigens were used to characterize IFN alpha producer cells. The production of IFN alpha was found to be a function of non-phagocytic, non-adherent, non-T, non-B, CD4+ (and to a lesser extent CD8+) MHC-class-II-positive cells. Furthermore, addition of anti-MHC (class II) mAb during PBMC incubation with virus-infected cells reduced IFN yields, suggesting that masking of these surface antigens alters PBMC responsiveness to IFN induction.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Cells, Cultured Coronaviridae/physiology Disease Susceptibility Histocompatibility Antigens/immunology,physiology Interferon Type I/metabolism Leukocytes, Mononuclear/metabolism,microbiology Swine/immunology T-Lymphocytes, Helper-Inducer/metabolism,microbiology Transmissible gastroenteritis virus/physiology
Chemicals
Antibodies, Monoclonal Histocompatibility Antigens Interferon Type I
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Charley B
Laboratoire de Virologie et d'Immunologie moléculaires, INRA, Jouy-en-Josas, France.
Lavenant L
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27 references, click to expand
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Article Info
Journal
Research in immunology
Abbr.
Res Immunol
ISSN
0923-2494
DOI
10.1016/0923-2494(90)90133-j
Published
1990-02-00
Pages
141-51
Language
English
Region
France
NLM ID
8907467
PMCID
PMC7135751
Subset
IM
External Links
PubMed source DOI: 10.1016/0923-2494(90)90133-j PMC Full Text
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