Home LiteratureArticle Details
PMID: 2166808 Published · ppublish English Journal Article

Polypeptide specificity of antiviral serum antibodies in children naturally infected with human rotavirus.

Journal of virology ·Vol. 64 ·No. 9 ·1990-09-00 ·Pages 4130-6

Brüssow H, Offit PA, Gerna G, Bruttin A, Sidoti J

Abstract

Reassortants between serotype 3 SA11 and serotype 6 NCDV rotaviruses were used to determine the relative amounts of serum-neutralizing antibody to VP4 and VP7 of serotype 3 SA11 rotavirus in children after natural rotavirus exposure. Sera from Ecuadorian children of a population-based study and sera from children of a hospital-based study in Germany (excluding diarrhea patients) demonstrated high titers of VP7-specific but only low titers of VP4-specific antibodies. In contrast, paired sera from German children hospitalized with a symptomatic primary rotavirus gastroenteritis demonstrated a titer increase to VP4 more frequently than to VP7 protein by neutralization test and immunoblotting. For these rotavirus patients, we provided, previously, direct evidence for the development of cross-neutralizing antibodies.

MeSH Terms
Antibodies, Viral/immunology,isolation & purification Antigens, Viral Capsid/immunology Capsid Proteins Child, Preschool Ecuador Germany, West Humans Immunoglobulin A/immunology,isolation & purification Immunoglobulin G/immunology,isolation & purification Immunoglobulin M/immunology,isolation & purification Infant Neutralization Tests Rotavirus/classification,immunology Rotavirus Infections/immunology Serotyping
Chemicals
Antibodies, Viral Antigens, Viral Capsid Proteins Immunoglobulin A Immunoglobulin G Immunoglobulin M VP4 protein, Rotavirus VP7 protein, Rotavirus
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Brüssow H
Nestlé Research Centre, Lausanne, Switzerland.
Offit P A
Gerna G
Bruttin A
Sidoti J
References (36)
36 references, click to expand
  1. Sequence analysis of the gene encoding the serotype-specific glycoprotein (VP7) of two new human rotavirus serotypes.
    Virology. 1989 Feb;168(2):429-33 PMID: 2536991
  2. Rotavirus VP7 neutralization epitopes of serotype 3 strains.
    Virology. 1989 Aug;171(2):503-15 PMID: 2474892
  3. Genes of human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralization and subgroup antigens.
    Virology. 1981 Jul 30;112(2):385-90 PMID: 6266134
  4. Gene coding assignments for growth restriction, neutralization and subgroup specificities of the W and DS-1 strains of human rotavirus.
    J Gen Virol. 1983 Feb;64 (Pt 2):313-20 PMID: 6300286
  5. Direct isolation in cell culture of human rotaviruses and their characterization into four serotypes.
    J Clin Microbiol. 1983 Aug;18(2):310-7 PMID: 6311872
  6. Serotyping of cell culture-adapted subgroup 2 human rotavirus strains by neutralization.
    Infect Immun. 1984 Feb;43(2):722-9 PMID: 6319289
  7. A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots.
    Anal Biochem. 1984 Jan;136(1):175-9 PMID: 6424501
  8. Serotypic similarity and diversity of rotaviruses of mammalian and avian origin as studied by plaque-reduction neutralization.
    J Infect Dis. 1984 May;149(5):694-702 PMID: 6202807
  9. Serotype determination of human rotavirus isolates and antibody prevalence in pediatric population in Hokkaido, Japan.
    Arch Virol. 1984;81(1-2):1-12 PMID: 6331342
  10. A candidate for a new serotype of human rotavirus.
    J Virol. 1985 May;54(2):623-4 PMID: 2985825
  11. Identification of the two rotavirus genes determining neutralization specificities.
    J Virol. 1986 Jan;57(1):376-8 PMID: 3001359
  12. Conservation of amino acid sequence of VP8 and cleavage region of 84-kDa outer capsid protein among rotaviruses recovered from asymptomatic neonatal infection.
    Proc Natl Acad Sci U S A. 1986 Sep;83(18):7039-43 PMID: 3018754
  13. Reassortant rotaviruses containing structural proteins vp3 and vp7 from different parents induce antibodies protective against each parental serotype.
    J Virol. 1986 Nov;60(2):491-6 PMID: 3021983
  14. Serum antibody responses to individual viral polypeptides in human rotavirus infections.
    J Gen Virol. 1987 Mar;68 ( Pt 3):643-51 PMID: 3029295
  15. Protection against severe rotavirus diarrhoea by rhesus rotavirus vaccine in Venezuelan infants.
    Lancet. 1987 Apr 18;1(8538):882-4 PMID: 2882289
  16. Cross-reactive neutralization epitopes on VP3 of human rotavirus: analysis with monoclonal antibodies and antigenic variants.
    J Virol. 1987 May;61(5):1726-30 PMID: 2437322
  17. Characterization of a second bovine rotavirus serotype.
    Arch Virol. 1987;94(1-2):29-41 PMID: 3034203
  18. Prevalent patterns of serotype-specific seroconversion in Mexican children infected with rotavirus.
    J Clin Microbiol. 1987 May;25(5):960-3 PMID: 3034971
  19. Development of a monoclonal antibody specific for serotype 3 rotavirus strains.
    Eur J Clin Microbiol. 1987 Apr;6(2):136-41 PMID: 3036497
  20. Immune response to rotavirus polypeptides after vaccination with heterologous rotavirus vaccines (RIT 4237, RRV-1).
    J Gen Virol. 1987 Jul;68 ( Pt 7):1993-9 PMID: 3037019
  21. Epitope-specific immune responses to rotavirus vaccination.
    Gastroenterology. 1987 Nov;93(5):941-50 PMID: 2443417
  22. Rotavirus isolate WI61 representing a presumptive new human serotype.
    J Clin Microbiol. 1987 Sep;25(9):1757-62 PMID: 2443534
  23. The rhesus rotavirus gene encoding protein VP3: location of amino acids involved in homologous and heterologous rotavirus neutralization and identification of a putative fusion region.
    Proc Natl Acad Sci U S A. 1988 Feb;85(3):645-9 PMID: 2829198
  24. Relative concentrations of serum neutralizing antibody to VP3 and VP7 proteins in adults infected with a human rotavirus.
    J Virol. 1988 May;62(5):1543-9 PMID: 3357205
  25. Prevalence of antibodies to rotavirus in different age-groups of infants in Bochum, West Germany.
    J Infect Dis. 1988 May;157(5):1014-22 PMID: 2834458
  26. Identification of cross-reactive and serotype 2-specific neutralization epitopes on VP3 of human rotavirus.
    J Virol. 1988 Jul;62(7):2421-6 PMID: 2453680
  27. Sequence of the fourth gene of human rotaviruses recovered from asymptomatic or symptomatic infections.
    J Virol. 1988 Aug;62(8):2978-84 PMID: 2839714
  28. Protective effect of WC3 vaccine against rotavirus diarrhea in infants during a predominantly serotype 1 rotavirus season.
    J Infect Dis. 1988 Sep;158(3):570-87 PMID: 2842405
  29. Seroconversion patterns to four human rotavirus serotypes in hospitalized infants with acute rotavirus gastroenteritis.
    J Infect Dis. 1988 Sep;158(3):588-95 PMID: 2842406
  30. Development of neutralizing antibodies and group A common antibodies against natural infections with human rotavirus.
    J Clin Microbiol. 1988 Aug;26(8):1506-12 PMID: 2844845
  31. Protection of Peruvian children against rotavirus diarrhea of specific serotypes by one, two, or three doses of the RIT 4237 attenuated bovine rotavirus vaccine.
    J Infect Dis. 1989 Mar;159(3):452-9 PMID: 2536789
  32. Complete nucleotide sequence of the simian rotavirus SA11 VP4 gene.
    Nucleic Acids Res. 1989 Mar 11;17(5):2122 PMID: 2538804
  33. Reactions to and antigenicity of two human-rhesus rotavirus reassortant vaccine candidates of serotypes 1 and 2 in Venezuelan infants.
    J Clin Microbiol. 1989 Mar;27(3):512-8 PMID: 2541168
  34. Molecular determinant of rotavirus neutralization and protection.
    Adv Virus Res. 1989;36:181-214 PMID: 2472045
  35. Complete nucleotide sequence of the gene encoding VP4 of a human rotavirus (strain K8) which has unique VP4 neutralization epitopes.
    J Virol. 1989 Sep;63(9):4101-6 PMID: 2474677
  36. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.9.4130-4136.1990
Published
1990-09-00
Pages
4130-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC247876
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.64.9.4130-4136.1990 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2