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PMID: 216633 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Rapid micromeasurement of neutrophil exocytosis.

Inflammation ·Vol. 3 ·No. 2 ·1978-06-00 ·Pages 129-35

Webster RO, Henson PM

Abstract

A simple method is described for the visual detection of neutrophil granule exocytosis. The microassay measures release of myeloperoxidase from azurophilic granules following neutrophil stimulation and can be accomplished in less than 30 min. The assay is sensitive, reproducible from day to day, and does not require separation of leukocytes from the reaction before enzyme assay. The procedure is also applicable to the rapid measurement of the suppression of degranulation by antiinflammatory agents.

MeSH Terms

Anti-Inflammatory Agents/pharmacology Exocytosis/drug effects Humans In Vitro Techniques Methods Neutrophils/drug effects,enzymology,physiology Peroxidase/blood

Chemicals

Anti-Inflammatory Agents Peroxidase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Webster R O

Henson P M

References (11)

11 references, click to expand

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Effect of anti-inflammatory drugs and agents that elevate intracellular cyclic AMP on the release of toxic oxygen metabolites by phagocytes: studies in a model of tissue-bound IgG.
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In vitro studies of immunologically induced secretion of mediators from cells and related phenomena.
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The structure-activity relations of synthetic peptides as chemotactic factors and inducers of lysosomal secretion for neutrophils.
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Stimulation of human neutrophils by soluble and insoluble immunoglobulin aggregates. Secretion of granule constituents and increased oxidation of glucose.
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Mechanisms of lysosomal enzyme release from human leukocytes: microtubule assembly and membrane fusion induced by a component of complement.
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2916-20 PMID: 4355374
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Article Info

Journal

Inflammation

Abbr.

Inflammation

ISSN

0360-3997

DOI

10.1007/BF00910734

Published

1978-06-00

Pages

129-35

Language

English

Region

United States

NLM ID

7600105

Subset

External Links

PubMed source DOI: 10.1007/BF00910734 View journal details

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