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PMID: 2165608 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

FrzE of Myxococcus xanthus is homologous to both CheA and CheY of Salmonella typhimurium.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 15 ·1990-08-00 ·Pages 5898-902

McCleary WR, Zusman DR

Abstract

Myxococcus xanthus exhibits multicellular development. The "frizzy" (frz) mutants are unable to complete the developmental pathway. Instead of forming fruiting bodies, these mutants form tangled filaments of cells. We have previously shown that four of the frz gene products are homologous to enteric chemotaxis proteins and have proposed that the frz genes constitute a signal-transduction pathway that controls the frequency at which cells reverse their gliding direction. We show here that frzE encodes a protein with a calculated molecular mass of 83 kDa. FrzE is homologous to both CheA and CheY of Salmonella typhimurium, which are members of a family of "two-component response regulators." It is thought that the modulator components autophosphorylate and transfer a phosphate group to their cognate effector components. FrzE contains an unusual (alanine plus proline)-rich region that might constitute a flexible hinge facilitating phosphate transfer between functional domains. We suggest that FrzE is a second messenger that relays information between the signaling protein FrzCD and the gliding motor.

MeSH Terms
Alanine Amino Acid Sequence Base Sequence DNA Transposable Elements DNA, Bacterial/genetics Escherichia coli/enzymology,genetics Genes, Bacterial Molecular Sequence Data Mutation Myxococcales/genetics Plasmids Proline Pyruvate Dehydrogenase Complex/genetics Restriction Mapping Salmonella typhimurium/genetics Sequence Homology, Nucleic Acid Signal Transduction
Chemicals
DNA Transposable Elements DNA, Bacterial Pyruvate Dehydrogenase Complex Proline Alanine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
McCleary W R
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Zusman D R
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26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.15.5898
Published
1990-08-00
Pages
5898-902
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54436
Subset
IM
Grants
NIGMS NIH HHS · GM 07232-12 · United States
NIGMS NIH HHS · GM 20509 · United States
Databases
GENBANK
M35192
External Links
PubMed source DOI: 10.1073/pnas.87.15.5898 PMC Full Text View journal details
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