Abstract
We have synthesized 32P-labeled hybridization probes from a hyperdivergent region (nucleotides 51 to 392) of the rotavirus gene encoding the VP7 glycoprotein by using the polymerase chain reaction method. Both RNA (after an initial reverse transcription step) and cloned cDNA from human rotavirus serotypes 1 through 4 could be used as templates to amplify this region. High-stringency hybridization of each of the four probes to rotavirus RNAs dotted on nylon membranes allowed the specific detection of corresponding sequences and thus permitted identification of the serotype of the strains dotted. The procedure was useful when applied to rotaviruses isolated from field studies.
MeSH Terms
Antigens, Viral/genetics
Base Sequence
Blotting, Northern
Capsid/genetics
Capsid Proteins
DNA, Viral/genetics
Feces/microbiology
Genes, Viral
Humans
Infant, Newborn
Molecular Sequence Data
Nucleic Acid Hybridization
Oligonucleotide Probes
Phosphorus Radioisotopes
Polymerase Chain Reaction/methods
RNA, Viral/genetics
Rotavirus/classification,genetics,isolation & purification
Serotyping
Viral Structural Proteins/genetics
Viral Vaccines
Chemicals
Antigens, Viral
Capsid Proteins
DNA, Viral
Oligonucleotide Probes
Phosphorus Radioisotopes
RNA, Viral
VP7 protein, Rotavirus
Viral Structural Proteins
Viral Vaccines
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Flores J
Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Sears J
Schael I P
White L
Garcia D
Lanata C
Kapikian A Z
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12 references, click to expand
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