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PMID: 2164605 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cloning of minute virus of mice cDNAs and preliminary analysis of individual viral proteins expressed in murine cells.

Journal of virology ·Vol. 64 ·No. 8 ·1990-08-00 ·Pages 3967-73

Clemens KE, Cerutis DR, Burger LR, Yang CQ, Pintel DJ

Abstract

cDNAs corresponding to RNA from the autonomous parvovirus minute virus of mice were cloned into constitutive and inducible expression vectors. These clones generate viral NS2, VP1, and VP2 proteins individually. Initial examination of these clones by transient expression analysis and analysis of stably transformed murine cell lines inducibly expressing these constructs indicated that they will be useful tools for characterizing the function of individual minute virus of mice gene products.

MeSH Terms
Animals Capsid/genetics Cell Line Cloning, Molecular/methods DNA, Viral/genetics Gene Expression Genetic Vectors Humans Mammary Tumor Virus, Mouse/genetics Mice Minute Virus of Mice/genetics Parvoviridae/genetics Phosphorylation Transcription Factors/genetics Transcription, Genetic Transfection Viral Core Proteins/genetics Viral Nonstructural Proteins Viral Proteins/analysis,genetics
Chemicals
DNA, Viral Transcription Factors Viral Core Proteins Viral Nonstructural Proteins Viral Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Clemens K E
Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri-Columbia 65212.
Cerutis D R
Burger L R
Yang C Q
Pintel D J
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.8.3967-3973.1990
Published
1990-08-00
Pages
3967-73
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249693
Subset
IM
Grants
NIAID NIH HHS · K04 AI-00934 · United States
NIAID NIH HHS · R01 AI-21302 · United States
NIAID NIH HHS · T32 AI-07276 · United States
External Links
PubMed source DOI: 10.1128/JVI.64.8.3967-3973.1990 PMC Full Text View journal details
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