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PMID: 2164590 Published · ppublish English Journal Article

Polypeptide composition of rotavirus empty capsids and their possible use as a subunit vaccine.

Journal of virology ·Vol. 64 ·No. 8 ·1990-08-00 ·Pages 3635-42

Brüssow H, Bruttin A, Marc-Martin S

Abstract

Two types of empty capsid particles that differed with respect to the presence of the two outer shell proteins were isolated from MA-104 cells infected with bovine rotavirus V1005. Three previously uncharacterized polypeptides, I, II, and III, migrating between VP2 and VP6, were detected in empty capsids but not in single- and double-shelled rotavirus particles. Peptide mapping revealed that all three proteins were related to VP2. Polypeptides I, II, and III could be generated by in vitro trypsin digestion of empty capsids not exposed to trypsin in the infection medium. Labeled polypeptides appeared in empty capsids before they were detected in intracellular single- or double-shelled rotavirus particles. Empty capsids were also observed in MA-104 cells infected with bovine rotaviruses UK and NCDV, simian rotavirus SA11, and human rotavirus KU. VP7-containing empty capsid is the minimal subunit vaccine for cows; we failed to induce a substantial neutralizing antibody increase with VP7 purified under denaturating or nondenaturating conditions or with synthetic peptides corresponding to two regions of VP7.

MeSH Terms
Animals Antigens, Viral/isolation & purification Capsid/biosynthesis,immunology,isolation & purification Cattle Cell Line Centrifugation, Zonal Electrophoresis, Polyacrylamide Gel Humans Macaca mulatta Macromolecular Substances Methionine/metabolism Rotavirus/analysis,immunology Serine Endopeptidases Trypsin Uridine/metabolism Viral Vaccines
Chemicals
Antigens, Viral Macromolecular Substances Viral Vaccines Methionine Serine Endopeptidases glutamyl endopeptidase Trypsin Uridine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brüssow H
Nestlé Research Centre, NESTEC Ltd., Vers-chez-les-Blanc, Lausanne, Switzerland.
Bruttin A
Marc-Martin S
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.8.3635-3642.1990
Published
1990-08-00
Pages
3635-42
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249656
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.64.8.3635-3642.1990 PMC Full Text View journal details
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