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PMID: 21620140 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Analysis of the human endogenous coregulator complexome.

Cell ·Vol. 145 ·No. 5 ·2011-05-27 ·Pages 787-99

Malovannaya A, Lanz RB, Jung SY, Bulynko Y, Le NT, Chan DW, Ding C, Shi Y, Yucer N, Krenciute G, Kim BJ, Li C, Chen R, Li W, Wang Y, O'Malley BW, Qin J

Abstract

Elucidation of endogenous cellular protein-protein interactions and their networks is most desirable for biological studies. Here we report our study of endogenous human coregulator protein complex networks obtained from integrative mass spectrometry-based analysis of 3290 affinity purifications. By preserving weak protein interactions during complex isolation and utilizing high levels of reciprocity in the large dataset, we identified many unreported protein associations, such as a transcriptional network formed by ZMYND8, ZNF687, and ZNF592. Furthermore, our work revealed a tiered interplay within networks that share common proteins, providing a conceptual organization of a cellular proteome composed of minimal endogenous modules (MEMOs), complex isoforms (uniCOREs), and regulatory complex-complex interaction networks (CCIs). This resource will effectively fill a void in linking correlative genomic studies with an understanding of transcriptional regulatory protein functions within the proteome for formulation and testing of future hypotheses.

MeSH Terms
Amino Acid Sequence BRCA1 Protein/metabolism Genome-Wide Association Study Humans Immunoprecipitation Mass Spectrometry Molecular Sequence Data Protein Interaction Mapping Proteins/metabolism Proteome/analysis Receptors, Cytoplasmic and Nuclear/metabolism Transcription, Genetic
Chemicals
BRCA1 Protein Proteins Proteome Receptors, Cytoplasmic and Nuclear
Authors & Affiliations
17 authors, click to expand affiliations / ORCID
Malovannaya Anna
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
Lanz Rainer B
Jung Sung Yun
Bulynko Yaroslava
Le Nguyen T
Chan Doug W
Ding Chen
Shi Yi
Yucer Nur
Krenciute Giedre
Kim Beom-Jun
Li Chunshu
Chen Rui
Li Wei
Wang Yi
O'Malley Bert W
Qin Jun
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
1097-4172
DOI
10.1016/j.cell.2011.05.006
Published
2011-05-27
Pages
787-99
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC3131083
Subset
IM
Grants
NIGMS NIH HHS · GM080703 · United States
NIDDK NIH HHS · DK059820 · United States
NIDDK NIH HHS · P01 DK059820 · United States
NIDDK NIH HHS · U19 DK062434 · United States
NIDDK NIH HHS · U19 DK062434-10 · United States
NIDDK NIH HHS · P01 DK059820-10 · United States
NCI NIH HHS · R01 CA084199 · United States
NIDDK NIH HHS · U19-DK62434 · United States
NICHD NIH HHS · F32 HD008188 · United States
NIGMS NIH HHS · R01 GM080703 · United States
NICHD NIH HHS · R01 HD007857 · United States
NCI NIH HHS · CA84199 · United States
NICHD NIH HHS · R01 HD008188 · United States
NICHD NIH HHS · R01 HD008188-40 · United States
NICHD NIH HHS · HD08188 · United States
External Links
PubMed source DOI: 10.1016/j.cell.2011.05.006 PMC Full Text View journal details
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