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PMID: 2161955 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Human cytomegalovirus immediate-early two protein region involved in negative regulation of the major immediate-early promoter.

Journal of virology ·Vol. 64 ·No. 7 ·1990-07-00 ·Pages 3532-6

Hermiston TW, Malone CL, Stinski MF

Abstract

The immediate-early two (IE2) gene products of human cytomegalovirus negatively regulate gene expression from the major immediate-early promoter in permissive human fibroblasts. A mutational analysis of the IE2 proteins indicated that the carboxyl-terminal region is required for negative regulation. The IE2 proteins that lack amino acid residues 365 to 519, or the carboxyl-terminal amino acids failed to negatively regulate. Most of the amino-terminal portion of the IE2 protein was not required for negative regulation. A possible explanation of the negative effect on downstream expression by the IE2 proteins is discussed.

MeSH Terms
Cloning, Molecular Cytomegalovirus/genetics DNA Mutational Analysis Gene Expression Regulation, Viral Genes, Viral Immediate-Early Proteins/physiology Membrane Glycoproteins Promoter Regions, Genetic RNA, Messenger/genetics RNA, Viral/genetics Restriction Mapping Structure-Activity Relationship Trans-Activators Viral Envelope Proteins Viral Proteins/genetics Viral Structural Proteins/genetics
Chemicals
IE2 protein, Cytomegalovirus Immediate-Early Proteins Membrane Glycoproteins RNA, Messenger RNA, Viral Trans-Activators UL115 protein, Human herpesvirus 5 Viral Envelope Proteins Viral Proteins Viral Structural Proteins glycoprotein H, Cytomegalovirus glycoprotein H, Human cytomegalovirus glycoprotein O, cytomegalovirus
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hermiston T W
Department of Microbiology, College of Medicine, University of Iowa, Iowa City 52242.
Malone C L
Stinski M F
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.7.3532-3536.1990
Published
1990-07-00
Pages
3532-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249624
Subset
IM
Grants
NIAID NIH HHS · AI-13562 · United States
External Links
PubMed source DOI: 10.1128/JVI.64.7.3532-3536.1990 PMC Full Text View journal details
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