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PMID: 2161951 Published · ppublish English Journal Article

Localization of the membrane-associated region of vesicular stomatitis virus M protein at the N terminus, using the hydrophobic, photoreactive probe 125I-TID.

Journal of virology ·Vol. 64 ·No. 7 ·1990-07-00 ·Pages 3486-91

Lenard J, Vanderoef R

Abstract

The membrane-reactive, photoactivatable probe 125I-TID [3-(trifluoromethyl)-3-(m-[125I]iodophenyl)-3H-diazirine] was found to label the M protein of vesicular stomatitis virus about 40% as much as G protein in intact virions, in agreement with labeling studies with other probes. By analyzing limited tryptic digestion and specific chemical cleavage products, the label was essentially entirely localized within the first 19, and probably within the first 5 to 10, amino acid residues at the N terminus, identifying this short amphipathic segment as the likely site of interaction of M protein with the viral bilayer.

MeSH Terms
Amino Acid Sequence Animals Azirines Binding Sites Cricetinae Formates Membrane Proteins/ultrastructure Molecular Sequence Data Peptide Mapping Photochemistry Solubility Trypsin Vesicular stomatitis Indiana virus/ultrastructure Viral Matrix Proteins/ultrastructure
Chemicals
Azirines Formates Membrane Proteins Viral Matrix Proteins formic acid 3-(trifluoromethyl)-3-(3-iodophenyl)diazirine Trypsin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lenard J
Department of Physiology and Biophysics, Robert Wood Johnson Medical School, University of Medicine and Dentistry, New Jersey, Piscataway 08854-5635.
Vanderoef R
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.7.3486-3491.1990
Published
1990-07-00
Pages
3486-91
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249613
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.64.7.3486-3491.1990 PMC Full Text View journal details
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