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PMID: 2160051 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Implications for the cis-requirements for Ds transposition based on the sequence of the wxB4 Ds element.

Molecular & general genetics : MGG ·Vol. 220 ·No. 3 ·1990-02-00 ·Pages 414-8

Varagona M, Wessler SR

Abstract

The nucleotide sequence of the 1494 bp wxB4 Ds element is presented. A comparison with previously characterized Ds elements reveals several novel features. This element has less Ac terminal sequence than other Ac-like Ds elements. The left terminus contains 398 bp of Ac sequence interrupted by a transposon-like DNA insertion, leaving only 317 bp of contiguous Ac sequence. The right terminus has 259 bp of Ac terminal sequence. The interior of the element contains sequences not found in other cloned members of the Ac/Ds family. We suggest that the role of this non-Ac DNA is to separate the Ac termini by a minimum distance and may be a cis requirement for Ds transposition in maize.

MeSH Terms
Base Sequence Blotting, Southern DNA/genetics DNA Transposable Elements Genes, Plant Molecular Sequence Data Repetitive Sequences, Nucleic Acid Restriction Mapping Sequence Homology, Nucleic Acid Zea mays/genetics
Chemicals
DNA Transposable Elements DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Varagona M
Botany Department, University of Georgia, Athens 30602.
Wessler S R
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23 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00391747
Published
1990-02-00
Pages
414-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM 32528 · United States
External Links
PubMed source DOI: 10.1007/BF00391747
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