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PMID: 2159284 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Hepatic uptake and metabolic disposition of leukotriene B4 in rats.

The Biochemical journal ·Vol. 267 ·No. 2 ·1990-04-15 ·Pages 467-70

Hagmann W, Korte M

Abstract

1. In isolated perfused rat liver and in vivo, up to 25% of [3H]leukotriene B4 was eliminated from the circulation via hepatic uptake and biliary excretion within 1 h. Total body recovery of 3H amounted to about 60% of infused [3H]leukotriene B4. 2. Hepatobiliary excretion of leukotriene B4 and its metabolites exceeded renal elimination by about 4-fold and depended, in contrast with excretion of cysteinyl leukotriene E4, upon continuous taurocholate supply. 3. Analyses of bile, liver and recirculated perfusate using h.p.l.c. indicated that the liver metabolized leukotriene B4 extensively to omega-carboxyleukotriene B4 and its beta-oxidized derivatives, and no unmetabolized leukotriene B4 appeared in bile. These results substantiate the important contribution of the hepatobiliary system with respect to the metabolic fate of leukotriene B4.

MeSH Terms
Animals Biological Transport Chromatography, High Pressure Liquid Kinetics Leukotriene B4/isolation & purification,metabolism Liver/metabolism Male Perfusion Rats Rats, Inbred Strains Tissue Distribution Tritium
Chemicals
Tritium Leukotriene B4
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hagmann W
Division of Tumor Biochemistry, Deutsches Krebsforschungszentrum, Heidelberg, Federal Republic of Germany.
Korte M
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46 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2670467
Published
1990-04-15
Pages
467-70
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1131312
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2670467 PMC Full Text View journal details
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