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PMID: 2158978 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Induction of the nag regulon of Escherichia coli by N-acetylglucosamine and glucosamine: role of the cyclic AMP-catabolite activator protein complex in expression of the regulon.

Journal of bacteriology ·Vol. 172 ·No. 5 ·1990-05-00 ·Pages 2728-35

Plumbridge JA

Abstract

The divergent nag regulon located at 15.5 min on the Escherichia coli map encodes genes necessary for growth on N-acetylglucosamine and glucosamine. Full induction of the regulon requires both the presence of N-acetylglucosamine and a functional cyclic AMP (cAMP)-catabolite activator protein (CAP) complex. Glucosamine produces a lower level of induction of the regulon. A nearly symmetric consensus CAP-binding site is located in the intergenic region between nagE (encoding EIINag) and nagB (encoding glucosamine-6-phosphate deaminase). Expression of both nagE and nagB genes is stimulated by cAMP-CAP, but the effect is more pronounced for nagE. In fact, very little expression of nagE is observed in the absence of cAMP-CAP, whereas 50% maximum expression of nagB is observed with N-acetylglucosamine in the absence of cAMP-CAP. Two mRNA 5' ends separated by about 100 nucleotides were located before nagB, and both seem to be similarly subject to N-acetylglucosamine induction and cAMP-CAP stimulation. To induce the regulon, N-acetylglucosamine or glucosamine must enter the cell, but the particular transport mechanism used is not important.

MeSH Terms
Acetylglucosamine/metabolism,pharmacology Aldose-Ketose Isomerases Base Sequence Binding Sites Biological Transport Carbohydrate Epimerases/genetics Carbohydrate Metabolism Cloning, Molecular Cyclic AMP Receptor Protein/metabolism Escherichia coli/drug effects,genetics,growth & development Gene Expression Genes, Regulator/drug effects Genotype Glucosamine/analogs & derivatives,metabolism,pharmacology Molecular Sequence Data Mutation Phosphoenolpyruvate Sugar Phosphotransferase System/genetics RNA, Messenger/genetics,isolation & purification Transcription, Genetic
Chemicals
Cyclic AMP Receptor Protein RNA, Messenger Phosphoenolpyruvate Sugar Phosphotransferase System glucosamine-6-phosphate isomerase Carbohydrate Epimerases Aldose-Ketose Isomerases Glucosamine Acetylglucosamine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Plumbridge J A
Institut de Biologie Physico-Chimique, Paris, France.
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.5.2728-2735.1990
Published
1990-05-00
Pages
2728-35
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208918
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.172.5.2728-2735.1990 PMC Full Text View journal details
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