Initial rates of pyruvate transport in mitochondria determined by an "inhibitor-stop" technique.

The Biochemical journal ·Vol. 150 ·No. 3 ·1975-09-00 ·Pages 553-6

Titheradge MA, Coore HG

Abstract

An "inhibitor-stop" technique has been developed for measuring initial rates of pyruvate transport into mitochondria. The technique uses alpha-cyano-3-hydroxycinnamate as the inhibitor and separates the mitochondria from the radioactive medium by Millipore filtration. Observed rates depend on availability of hydroxyl and other exchangeable anions within the mitochondrial matrix.

MeSH Terms

Animals Biological Transport/drug effects Cinnamates/pharmacology Coumaric Acids/pharmacology Hydrogen-Ion Concentration Male Mitochondria, Liver/drug effects,metabolism Nitriles/pharmacology Pyruvates/analysis,metabolism Rats Temperature Time Factors

Chemicals

Cinnamates Coumaric Acids Nitriles Pyruvates

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Titheradge M A

Coore H G

References (11)

11 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1500553

Published

1975-09-00

Pages

553-6

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1165770

Subset

External Links

PubMed source DOI: 10.1042/bj1500553 PMC Full Text View journal details

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Initial rates of pyruvate transport in mitochondria determined by an "inhibitor-stop" technique.

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