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PMID: 21541037 Published · epublish English Journal Article

Identification of receptor tyrosine kinase, discoidin domain receptor 1 (DDR1), as a potential biomarker for serous ovarian cancer.

International journal of molecular sciences ·Vol. 12 ·No. 2 ·2011-01-31 ·Pages 971-82

Quan J, Yahata T, Adachi S, Yoshihara K, Tanaka K

Abstract

Ovarian cancer, one of the most common gynecological malignancies, has an aggressive phenotype. It is necessary to develop novel and more effective treatment strategies against advanced disease. Protein tyrosine kinases (PTKs) play an important role in the signal transduction pathways involved in tumorigenesis, and represent potential targets for anticancer therapies. In this study, we performed cDNA subtraction following polymerase chain reaction (PCR) using degenerate oligonucleotide primers to identify specifically overexpressed PTKs in ovarian cancer. Three PTKs, janus kinase 1, insulin-like growth factor 1 receptor, and discoidin domain receptor 1 (DDR1), were identified and only DDR1 was overexpressed in all ovarian cancer tissues examined for the validation by quantitative real-time PCR. The DDR1 protein was expressed in 63% (42/67) of serous ovarian cancer tissue, whereas it was undetectable in normal ovarian surface epithelium. DDR1 was expressed significantly more frequently in high-grade (79%) and advanced stage (77%) tumors compared to low-grade (50%) and early stage (43%) tumors. The expression of the DDR1 protein significantly correlated with poor disease-free survival. Although its functional role and clinical utility remain to be examined in future studies, our results suggest that the expression of DDR1 may serve as both a potential biomarker and a molecular target for advanced ovarian cancer.

Keywords
DDR1 cDNA subtraction degenerate PCR disease biomarker ovarian cancer tyrosine kinase
MeSH Terms
Biomarkers, Tumor/genetics,metabolism Cystadenoma/diagnosis,metabolism Discoidin Domain Receptor 1 Female Humans Janus Kinase 1/genetics,metabolism Ovarian Neoplasms/diagnosis,metabolism Prognosis Receptor Protein-Tyrosine Kinases/genetics,metabolism Receptor, IGF Type 1/genetics,metabolism Serous Membrane/metabolism,pathology
Chemicals
Biomarkers, Tumor DDR1 protein, human Discoidin Domain Receptor 1 Receptor Protein-Tyrosine Kinases Receptor, IGF Type 1 JAK1 protein, human Janus Kinase 1
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Quan Jinhua
Division of Obstetrics and Gynecology, Niigata University, Graduate School of Medical and Dental Sciences, Niigata, 951-8520, Japan; E-Mails: zenkinka@yahoo.co.jp (J.Q.); sadachi@med.niigata-u.ac.jp (S.A.); yoshikou@med.niigata-u.ac.jp (K.Y.); tanaken@med.niigata-u.ac.jp (K.T.).
Yahata Tetsuro
Adachi Sosuke
Yoshihara Kosuke
Tanaka Kenichi
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Article Info
Journal
International journal of molecular sciences
Abbr.
Int J Mol Sci
ISSN
1422-0067
DOI
10.3390/ijms12020971
Email
zenkinka@yahoo.co.jp
sadachi@med.niigata-u.ac.jp
yoshikou@med.niigata-u.ac.jp
tanaken@med.niigata-u.ac.jp
Published
2011-01-31
Epub
2011-00-31
Pages
971-82
Language
English
Region
Switzerland
NLM ID
101092791
PMCID
PMC3083684
Subset
IM
External Links
PubMed source DOI: 10.3390/ijms12020971 PMC Full Text View journal details
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